High Throughput Antibody Production for Phage Display-mediated Antibody Discovery

Creative Biolabs' high-throughput phage display antibody technology integrates genotype and phenotype and is capable of simulating the process of antibody production both in vivo and in vitro, offering a straightforward and rapid approach for the preparation of genetically engineered antibodies for various types of recombinant antibodies.

Services

Creative Biolabs offers the construction and screening services of phage display antibody libraries. Phage display refers to the preparation of antibody libraries by presenting scFv single-chain antibodies or Fab on the phage surface. The antibody library, when combined with solid-phase technology and high-throughput screening, can readily and rapidly acquire the desired antibody. The phage display library solution we provide not only offers a capacity of up to 108 antibody libraries but also provides antibody library construction services based on customer requirements.

Fig.1 High Throughput Antibody Phage Display Solutions. (Creative Biolabs Original)

Nowadays, the phage antibody library is classified into four main types:

The naive antibody library is genetically derived from B lymphocytes in human or animal blood, bone marrow, spleen, and amygdala. It can obtain human antibodies, and target all natural antigens, and the library is sufficiently large to obtain high-affinity antibodies directly.

The semi-synthetic antibody library is an antibody library based on the framework region and CDR1 and CDR2 regions of the natural antibody library, and the random synthesis and amplification of the DNA sequence in the CDR3 region. We can achieve the randomization of the CDR3 area to enhance the library capacity.

The synthetic antibody library is a purely synthetic antibody library based on antibody gene bank information. The fully synthetic antibody library can attain a high degree of randomization, resulting in an enormous library capacity.

The immune antibody library can also be constructed using the spleen of an animal immunized with a specific antigen, which can be more targeted to screen antibodies against a specific antigen.

Antibody Mouse Platform

Based on years of research and development experience in antibody drugs, Creative Biolabs has established a novel antibody development technology platform based on self-developed fully human antibody mice and human antibody phage libraries and is dedicated to offering customers rapid and efficient antibody development services.

Fig.2 Antibody Mouse Platform Development Process. (Creative Biolabs Original)

Delivery criteria: phage libraries, 1-2 antibody sequences; immunized animal sera, hybridoma cell supernatants, hybridoma cells, and 1-2 antibody sequences.

Customer information: antigen information, immune antigens, and antigens utilized for testing.

Advantages

• Highly humanized antibody mice available in the market;
• Extensive experience in animal immunization;
• Mature technology for hybridoma cell preparation;
• High-capacity and high throughput human antibody phage display libraries;
• Rich experience in the construction of phage libraries;
• High-affinity antibody development;
• Rich experience in the efficacy evaluation of antibody drugs;

Creative Biolabs provides high throughput phage library construction and antibody screening services covering a variety of library types and can provide customers with multi-species monoclonal antibody preparation services based on our phage antibody library platform technology. We can also provide customized services according to the needs of our customers and provide flexible and effective screening analysis solutions. If you are interested in our services, please feel free to contact us.

For research use only. Not intended for any clinical use.