High Throughput Antibody Production for Single B Antibody Discovery

Creative Biolabs provides comprehensive single B cell antibody preparation services, with the advantages of fast delivery, high throughput, high specificity, and affinity, covering the complete process from immunogen design to antibody production and corresponding detection method development, to ensure that customers can provide high-quality antibody solutions.

Platform

As a new generation of antibody development technology, single B cell antibody technology can efficiently and rapidly isolate antibodies from single B cells, which is another breakthrough in antibody generation technology after hybridoma and phage display. Based on the single B cell screening platform, Creative Biolabs has advantages from screening time to obtaining high-quality antibodies and can provide one-stop technical services from antigen design, synthesis, and modification to animal immunity, single B cell enrichment and screening, expression and purification, functional verification to meet different needs of customers.

Currently, we can use single B cell screening technology to prepare monoclonal antibodies of rabbits, mice, camel, sheep, chicken, and other species, and the antibodies obtained by single B cell technology have outstanding Features such as high specificity, high activity, and high affinity.

Fig.1 Single B Cell Screening Methods.^1,2

Chief Services

Creative Biolabs provides corresponding antibody preparation services for different species and can obtain sequence information through antibody sequencing. Our integrated service also offers customers better choices to meet their needs and helps you obtain high-quality antibodies quickly.

• Rabbit single B cell monoclonal antibody preparation services
• Mouse single B cell monoclonal antibody preparation services
• Monoclonal antibody preparation services
• Alpaca single B cell monoclonal antibody preparation services
• Antibody purification services
• Antibody expression and validation platform
• Antibody sequencing platform
• Recombinant antibody preparation services

Fig.2 High Throughput Single B Cell Screening Process. (Creative Biolabs Original)

Why Choose Us?

After years of optimization of antibody production technology, Creative Biolabs has completed the construction of a single B cell antibody discovery platform. Our single B rapid antibody discovery service has achieved full coverage of rich species such as mice, rabbits, alpacas, and fully human transgenic mice. In our labs, we use unique cell sorting and enrichment techniques to screen antibody clones from specific to functional to ensure the assay results accuracy. Till now, our high-throughput single B cell platform can reduce the workload of downstream expression validation and shorten the entire antibody discovery timeline, saving valuable time for antibody drug development.

• High efficiency: the fastest delivery in one month, five months less than hybridoma.
• High delivery: can guarantee delivery of at least 40 positive clones and ensure diversity, and hundreds of reserved clones;
• Double screening protection: memory B cell & plasma cell double screening system;
• Natural maturation: natural pairing of heavy and light chains, in vivo natural maturation of monoclonal antibodies;
• Recombinant stability: directly obtain recombinant expression plasmid, efficient and stable;
• Proprietary antibody big data platform to screen the most likely complete antibody sequences;
• Unique internal data capture tools to pre-process antibody sequencing results to reduce false positives;
• Complete bioinformatics analysis, experienced antibody research team, and in-depth mining of high-value information.

Creative Biolabs has rich experience in single B cell antibody preparation and can provide you with high purity of different forms of antibody products. Moreover, we also offer customers a variety of scale antibody testing services, with our perfect antibody purification platform, can deliver customers with high-quality customized antibody products in a short time. If you are interested in our services, please feel free to contact us.

References

1. Pedrioli, Alessandro, and Annette Oxenius. "Single B cell technologies for monoclonal antibody discovery." Trends in immunology 42.12 (2021): 1143-1158.
2. under Open Access License CC BY 4.0, without modification.

For research use only. Not intended for any clinical use.