High Throughput Antibody Production Service assist Transgenic Mice based Lead Discovery
Are you currently facing long drug development cycles, difficulty in obtaining high-quality recombinant proteins, or challenges in antibody development? Our high-throughput antibody production assists transgenic mice-based lead discovery, helps you accelerate drug discovery, and develop highly specific antibodies through innovative transgenic mouse models and advanced high-throughput screening platforms.
Introduction
The development of therapeutic antibodies has revolutionized disease treatment, yet conventional discovery methods are often time-consuming and prone to issues like immunogenicity. Transgenic mice engineered to produce human antibodies in vivo offer a superior solution by providing natural affinity maturation and reducing immunogenicity risks. Recent advancements in trans-chromosomic mouse models demonstrate stable maintenance of entire human immunoglobulin loci, leading to authentic human antibody repertoires and highly efficient hybridoma production, thereby accelerating the identification of high-quality therapeutic leads.
Service
At Creative Biolabs, our high-throughput antibody production platform, powered by advanced transgenic mouse technology, is designed to directly address the critical challenges in modern biopharmaceutical development. We provide specific, high-quality deliverables that streamline your lead discovery process and ensure the optimal characteristics of your therapeutic candidates.
- Accelerated Lead Identification: Our workflow screens hundreds to thousands of clones in parallel, cutting discovery timelines and speeding lead selection.
- Fully Human Candidates: Transgenic mouse models generate fully human antibodies in vivo, eliminating humanization and reducing immunogenicity risks.
- High Affinity & Specificity: Natural affinity maturation plus high-throughput screening delivers antibodies with superior binding and target specificity.
- Comprehensive Characterization: Each antibody is validated for purity, concentration, and function, ensuring readiness for preclinical and clinical use.
What We Can Offer
| Transgenic Animal Models | |
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| Downstream Antibody Engineering and Characterization | High-Throughput Antibody Discovery Platforms |
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Service Process
Key Advantages
- Integrated High-Throughput Workflow: From immunization to characterization, our automated systems deliver diverse antibodies rapidly, at scale, and with consistency - shortening timelines.
- Proven Track Record: With 20+ years of experience and thousands of projects delivered, we help clients achieve higher success rates and more developable leads.
- Comprehensive Quality Control: Each antibody is rigorously tested for purity, functionality, and developability, minimizing downstream risks and costs.
Representative Data
Summary: Trans-chromosomic mice with engineered chromosomes holding full human immunoglobulin heavy and kappa chain loci, in a mouse immunoglobulin-knockout background, are established. They stably maintain the chromosomes. High-throughput sequencing finds that they well mirror the human immunoglobulin repertoire. Despite slightly altered B cell development and immune response, they have more antigen-specific plasmablast/plasma cell subsets than wild-type mice, enabling efficient hybridoma production.
Fig.1 Generation of fully human antibody mice.1
FAQs
How does your service ensure high quality and specificity of antibodies?
We leverage advanced transgenic mouse models for in vivo affinity maturation, which naturally select for high-affinity and specific binders. Coupled with our high-throughput functional screening platforms and rigorous quality control measures.
What is the primary advantage of using transgenic mice over other antibody discovery platforms like phage display?
While phage display is powerful, transgenic mice offer the advantage of in vivo antibody development, leading to fully human antibodies that undergo natural affinity maturation and class-switching. This typically results in antibodies with superior developability characteristics, better solubility, and reduced immunogenicity risks compared to in vitro-generated antibodies.
What types of targets can your platform handle, including challenging ones?
Our platform is highly versatile and can generate antibodies against a wide range of targets, including membrane proteins, ion channels, and other difficult-to-express or weakly immunogenic antigens.
Why Choose Us?
Choosing Creative Biolabs for your high-throughput antibody production assists transgenic mice-based lead discovery, meaning partnering with a leader dedicated to accelerating your therapeutic development with unparalleled quality and efficiency. Our unique blend of cutting-edge technology, deep scientific expertise, and client-centric approach sets us apart.
Contact Us for further information and to go through your project.
Reference
- Satofuka, Hiroyuki et al. “Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci.” Nature Communications vol. 13,1 1841. 5 Apr. 2022. Distributed under Open Access License CC BY 4.0, without modification. https://doi.org/10.1038/s41467-022-29421-2
For research use only. Not intended for any clinical use.
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