Human Anti-IL4R (clone 12B5) scFv-Fc Chimera
CAT#: VS-0425-FY132
The anti-IL4R scFv-FC is a recombinant protein that targets the interleukin-4 receptor (IL4R), which plays a crucial role in mediating immune responses and inflammation. By binding to IL4R, this engineered single-chain variable fragment (scFv) fused with the Fc region of IgG can inhibit IL-4 signaling pathways, potentially providing therapeutic benefits in diseases such as asthma, allergic responses, and other IL-4 related conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm, plasma membrane & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70380/1812_F10_5_cr59ef13a97c5ff_selected.jpg
Normal Tissue
Figure 2 Tonsil
High expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in subsets of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4451/ihc_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/123264_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/123264_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HBEC3-KT, HDLM-2, U-698)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077238-IL4R
❮
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Specifications
- Immunogen
- A soluble human IL-4 receptor polypeptide.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human IL4R
- Species Reactivity
- Human
- Clone
- 12B5
- Applications
- Blocking
- Related Disease
- Ige Responsiveness, Atopic and Human Immunodeficiency Virus Type 1
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Interleukin 4 Receptor
- Gene ID
- 3566
- UniProt ID
- P24394
- Long Name
- Interleukin 4 Receptor
- Sequence Similarities
- Belongs to the type I cytokine receptor family. Type 4 subfamily.
- Cellular Localization
- Secreted
- Post Translation Modifications
- On IL4 binding, phosphorylated on C-terminal tyrosine residues.
Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.
The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
Glycosylation at Asn53, Asn98, Asn128, Asn134, Asn176, and Asn209
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect IL4RA_HUMAN (2053)
Glycosylation from GlyGen 6 sites, 4 N-linked glycans (2 sites)
- Protein Refseq
- XP_005255365.1; XP_011544127.1; XP_011544128.1; XP_011544129.1; XP_011544130.1
- Function
- Receptor for both interleukin 4 and interleukin 13.
Couples to the JAK1/2/3-STAT6 pathway.
The IL4 response is involved in promoting Th2 differentiation.
The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.
In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. IL4RA_HUMAN,P24394
Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| TAB-0004CL-S(P) | Anti-Human IL4R Recombinant Antibody scFv Fragment (12B5) | Inhib, RIA, ELISA, Block | Human antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1432CQ | Mouse Anti-IL4R Recombinant Antibody (clone CBL439) | WB, FC, IHC, CyTOF, Neut | Mouse IgG2a |
| NEUT-1433CQ | Mouse Anti-IL4R Recombinant Antibody (clone MM0405-4Y44) | WB, Neut, IHC, IHC-P | Mouse IgG1 |
| NEUT-1434CQ | Rabbit Anti-IL4R Recombinant Antibody (clone CBL1006) | Neut | Rabbit IgG |
| NEUT-1435CQ | Rabbit Anti-IL4R Recombinant Antibody (clone CBL998) | Neut | Rabbit IgG |
| NEUT-1436CQ | Mouse Anti-IL4R Recombinant Antibody (clone CBL075) | ELISA, Neut | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4007 | Rabbit Anti-Il4r Recombinant Antibody (clone SI69DS) | ELISA | Rabbit IgG |
| MOR-4274 | Rabbit Anti-IL4R Recombinant Antibody (clone SI357DS) | FC | Rabbit IgG |
| MOR-4275 | Rabbit Anti-IL4R Recombinant Antibody (clone SI358DS) | Neut | Rabbit IgG |
| MOR-4276 | Rabbit Anti-IL4R Recombinant Antibody (clone SI359DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0291-YC-S(P) | Human Anti-IL4R Recombinant Antibody (clone Antibody 1); scFv Fragment | HTRF, FuncS | Human scFv |
| HPAB-N0152-YC-S(P) | Mouse Anti-IL4R Recombinant Antibody; scFv Fragment (HPAB-N0152-YC-S(P)) | ELISA, Inhib, FuncS | Mouse scFv |
| HPAB-1755-FY-F(E) | Human Anti-IL4R Recombinant Antibody; Fab Fragment (HPAB-1755-FY-F(E)) | ELISA | Human Fab |
| HPAB-1756-FY-F(E) | Human Anti-IL4R Recombinant Antibody; Fab Fragment (HPAB-1756-FY-F(E)) | ELISA | Human Fab |
| HPAB-1757-FY-F(E) | Human Anti-IL4R Recombinant Antibody; Fab Fragment (HPAB-1757-FY-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0291-YC-F(E) | Human Anti-IL4R Recombinant Antibody (clone Antibody 1); Fab Fragment | HTRF, FuncS | Human Fab |
| HPAB-1447LY-F(E) | Mouse Anti-IL4R Recombinant Antibody; Fab Fragment (HPAB-1447LY-F(E)) | FC, ELISA, Block | Mouse Fab |
| HPAB-1448LY-F(E) | Mouse Anti-IL4R Recombinant Antibody; Fab Fragment (HPAB-1448LY-F(E)) | FC, ELISA, Block | Mouse Fab |
| HPAB-1449LY-F(E) | Mouse Anti-IL4R Recombinant Antibody; Fab Fragment (HPAB-1449LY-F(E)) | FC, ELISA, Block | Mouse Fab |
| HPAB-1450LY-F(E) | Mouse Anti-IL4R Recombinant Antibody; Fab Fragment (HPAB-1450LY-F(E)) | FC, ELISA, Block | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0344LY | Human Anti-IL4R Recombinant Antibody (HPAB-0344LY) | Block, ELISA | Human IgG1 |
| HPAB-0345LY | Human Anti-IL4R Recombinant Antibody (HPAB-0345LY) | Block, ELISA | Human IgG1 |
| HPAB-0346LY | Human Anti-IL4R Recombinant Antibody (HPAB-0346LY) | Block, ELISA | Human IgG1 |
| HPAB-0347LY | Human Anti-IL4R Recombinant Antibody (HPAB-0347LY) | Block, ELISA | Human IgG1 |
| HPAB-0348LY | Human Anti-IL4R Recombinant Antibody (HPAB-0348LY) | Block, ELISA | Human IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0425-YC103 | Recombinant Anti-IL4R Vesicular Antibody, EV Displayed (VS-0425-YC103) | ELISA, FC, Cell-uptake |
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