Human Anti-ROBO1 scFv-Fc Chimera (VS-0125-FY52) (CAT#: VS-0125-FY52)
Anti-ROBO1 scFv-Fc is a recombinant protein commonly used in biomedical research, particularly in studies related to cancer and neurological disorders. ROBO1 (Roundabout Guidance Receptor 1) is a membrane receptor that participates in cell migration and neuronal guidance, and it has been found to be associated with the progression of various cancers. Research on anti-ROBO1 scFv-Fc can help elucidate the role of ROBO1 in multiple diseases and may provide new insights for the treatment of related conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52968/if_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52968/if_selected.jpg
Figure 2 IHC staining of human breast
(Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/ihc_selected.jpg
(Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_7_3.jpg
Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13524/33705_A_9_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169855-ROBO1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169855-ROBO1
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Species Reactivity
- Human
- Applications
- Immunoassay
- Related Disease
- Cancer and neurological disorders
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Roundabout Guidance Receptor 1; Deleted In U Twenty Twenty; DUTT1; Roundabout, Axon Guidance Receptor, Homolog 1 (Drosophila); Roundabout (Axon Guidance Receptor, Drosophila) Homolog 1; Roundabout, Axon Guidance Receptor, Homolog 1; Roundabout Homolog 1; H-Robo-1; SAX3
- Gene ID
- 6091
- UniProt ID
- Q9Y6N7
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. ROBO family.
- Cellular Localization
- Cell membrane; Single-pass type I membrane protein
Cell projection, axon
Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass membrane protein
- Post Translation Modifications
- Ubiquitinated.
May be deubiquitinated by USP33.
Glycosylation at Asn160, Asn463, Thr660, Ser661, Asn790, Asn820, and Asn827
Ubiquitination at Lys1051
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 10 sites, 5 N-linked glycans (2 sites), 3 O-linked glycans (5 sites)
- Protein Refseq
- XP_006713340.1; XP_011532278.1; XP_011532279.1; XP_011532280.1; XP_011532281.1
- Function
- Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.
Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
ScFv Antibody Products
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Mouse Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-346 | Mouse Anti-ROBO1 Recombinant Antibody (PABL-346) | WB, ELISA, FuncS | Mouse IgG2a kappa |
| MOB-1275MZ | Recombinant Mouse Anti-Human ROBO1 Antibody (clone 2G6) | WB | Mouse antibody |
| MRO-2166-CN | Rabbit Anti-ROBO1 Polyclonal Antibody (MRO-2166-CN) | IF, IHC | Rabbit IgG |
| PABJ-0064 | Mouse Anti-ROBO1 Recombinant Antibody (PABJ-0064) | ELISA, FuncS | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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