Human Anti-SMAD4 scFv-Fc Chimera (VS-0325-FY201) (CAT#: VS-0325-FY201)

• Immunogen
• Peptides derived from SMAD4

• Host Species
• Human

• Type
• Human IgG1, scFv-Fc

• Specificity
• Human SMAD4

• Species Reactivity
• Human

• Applications
• ELISA, Immunofluorescence

• Purity
• >95% as determined by SDS-PAGE

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Alternative Names
• SMAD Family Member 4; Deletion Target In Pancreatic Carcinoma 4; MAD Homolog 4; MADH4; DPC4; MAD, Mothers Against Decapentaplegic Homolog 4 (Drosophila); Mothers Against Decapentaplegic, Drosophila, Homolog Of, 4; SMAD, Mothers Against DPP Homolog 4 (Drosophila); Mothers Against Decapentaplegic Homolog 4

• Gene ID
• 4089

• UniProt ID
• Q13485

• Long Name
• SMAD Family Member 4

• Sequence Similarities
• Belongs to the dwarfin/SMAD family.

• Cellular Localization
• Cytoplasm; Nucleus

• Post Translation Modifications
• Phosphorylated by PDPK1.
  Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination by USP9X restores its competence to mediate TGF-beta signaling.

• Protein Refseq
• NP_005350.1

• Function
• In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity).
  Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling (PubMed:25514493).
  Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Isotype Control

• MOB-200CQ
• Human IgG Fc Isotype Control Antibody

Secondary Antibody

• 2ND-029CQ
• Goat Anti-Human IgG Fc Antibody

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