IgG Elution Buffer (VS-0824-WK28)
CAT#: VS-0824-WK28
This IgG elution buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins (Protein A, Protein G, Protein A/G), including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.
Specifications
- Product Size
- 1 L
- Format
- Liquid
- Contents
- pH 2.8; amine-based
- Specifications
- Reagent type: elution buffer
pH: 2.8
- Storage
- Store at 4°C.
- Background
- This elution buffer ensures high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.
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High Recovery Rate
This elution buffer is excellent. We used it for eluting human IgG from Protein A columns and obtained a much higher recovery rate compared to the standard glycine-HCl buffer we used to use. It seems to be very effective at dissociating the antibody.
Preserves Antibody Activity
The best part about this buffer is that it is gentle on our antibodies. We noticed that our purified antibodies retained better binding activity after elution, likely because this formulation prevents the denaturation that can happen with harsher buffers.
Q&As
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What is the main benefit of using this specific IgG elution buffer?
A: This buffer is formulated to be gentle on antibodies while ensuring high recovery rates. Unlike harsh acidic buffers that can denature proteins or cause aggregation, this product is optimized to dissociate IgG from Protein A or G columns while preserving the antibody's native structure and biological activity.
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Is this elution buffer compatible with different IgG subtypes?
A: Yes, this buffer is designed to be broadly compatible with various IgG subtypes from different species (e.g., human, mouse, rabbit). It effectively disrupts the interaction between the Fc region and the affinity ligand (Protein A or G) across a wide range of antibody variants.
View the frequently asked questions answered by Creative Biolabs Support.
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