Magic™ Yeast Expression Service

The creative Magic™ Yeast Expression Service of Creative Biolabs remarkably possesses multiple superior technologies for the recombinant expression of pharmaceutical antibodies and other protein targets with the incomparable quantity and quality in the world. Our service is composed of all steps in the whole producing procedure. For instance, we have built an unparalleled library possessing almost every suitable expression cassette, hundreds of secretion leader sequences, and a number of well-established strains. Notably, the sophisticatedly designed technical strategy for particular target holds the most valuable core of the Magic™ Yeast Expression Service.

Magic™ Yeast Expression Service

The Introduction and Application of Yeast Expression

As a remarkably economical eukaryotic expression host, the yeast is able to add post-translational glycosylation with more powerful folding aids than prokaryotic E. coli. Meanwhile, further advanced genetic engineering has solved the vicious disadvantage of different glycosylation with the complex human N-glycan. Furthermore, the yeast harbors both intracellular and secretion routes, which leads to a more stable product with easier isolation and higher purity. As for the general characteristics, yeast grows rapidly in a slightly resource-intensive medium and it is easy to handling and manipulation under clear genetic background. Thus, the scientific realm has applied these incredible microorganisms to produce valuable antibodies, bio-functional proteins, sugars, and enzymes to fuel the basic research and industrial application.

Advantages of Magic™ Yeast Expression Service

  1. Excellent success rate for numerous proteins and antibodies of various species.
  2. The whole set of libraries for the initiation of creative expression, which include an astonishing plasmid library to satisfy almost every requirement (various promoters, selective markers, N- or C-terminal tags, soluble helpers, etc.) and a wonderful host strain library composed of standard expression strain, protein expression enhanced strain, toxic tolerant strain, etc.
  3. Optimal cultivation and induction with the whole range of scales from 5 mL to 1000 L per batch.
  4. Fabulous average yield up to 15 grams per liter with stable exopressional clones.
  5. One-stop service: from initial design and optimization all the way down to purification and further modification with well recorded data and organized report.

The Magic™ Yeast Expression Service has been dedicated to developing this conventional system into a whole new level of advancement. Our service has successfully delivered an unbelievable yield up to 15 grams per liter culture and the records are constantly broken by our well-designed expression strategies. Meanwhile, varied expression levels are also achievable for toxic proteins via sophisticated copy number control. Generally, the verified shuttle construct is multiplied in E. coli first. Then the linearized plasmid is transformed into particular strains via multiple transformation techniques. The subsequent integration on the yeast chromosome generates numerous stable expressional clones under a single or a combination of selection markers. Further empirical screen and scalable fermentation produce the desired target. Eventually, a well-designed downstream purification offers the superior product with optional characteristics upon requirement.

Magic™ Yeast Expression Service

Our service is dedicated to offering the best expression strategy in the world. Almost every suitable yeast species or strains are available upon our service, including the most popular Saccharomyces cerevisiae and Pichia pastoris. Furthermore, some minor species are also developed including Candida boidinii, Hansenula polymorpha, Pichia angusta, Kluyveromyces lactis, Yarrowia lipolytica, Arxula adeninivorans, etc. At Creative Biolabs, our Magic™ Yeast Expression Service offers the most superior yeast service for every valuable biopharmaceutical in the world.

Reference

  1. Dagar, Vikas Kumar, and Yogender Pal Khasa. "Yeast Expression Systems: Current Status and Future Prospects." Yeast Diversity in Human Welfare. Springer Singapore, 2017. 215-250.

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