Quick™ Magnetic Antibody Labeling Kit (VS-0824-FY98) (CAT#: VS-0824-FY98)
The magnetic antibody labeling kit offers a quick and efficient way to conjugate antibodies to specially treated magnetic particles. With a high binding capacity, covalent attachment, and no need for extensive optimization, it is ideal for immunoprecipitation experiments. The kit is easy to use, scalable, and has a long shelf life. Overall, it provides a stable and reliable method for antibody conjugation.
Specific Inquiry
Specifications
- Fluorescent Dye Name
- Magnetic
- Application
- Immunoprecipitation
- Composition
- Mag antibody diluent
Mag quencher
Mag storage buffer
Mag wash buffer
Magnetic particles vial
- Procedure Steps
- 1. Allow all reagents to come to room temperature before beginning.
2. Prepare a 0.4 mg/mL solution of your stock antibody by diluting it with mag antibody diluent.
3. Mix 50 μL of the 0.4 mg/mL antibody with the 20 µg vial, or 500 μL with the 200 µg vial, and gently pipette up and down to reconstitute the magnetic particles. Transfer the particles from the 20 µg vial into a microcentrifuge tube. Divide the particles from the 200 µg vial into two tubes (250 µL each) and incubate for 30 minutes at room temperature with constant mixing.
4. Use a magnetic stand for 5-10 seconds to collect the particles and remove the supernatant.
5. Add 200 μL of mag wash buffer to the tube for the 20 µg scale or 1 mL to each tube for the 200 µg scale, mix thoroughly for 15 seconds, and use a magnetic stand to collect the particles and discard the supernatant quickly due to the low pH of the buffer.
6. Repeat the washing step.
7. Add 200 μL of mag quencher to the tube for the 20 µg scale or 1 mL to each tube for the 200 µg scale, mix thoroughly, and incubate for 30 minutes at room temperature with constant mixing. Use a magnetic stand for 5-10 seconds to collect the particles and discard the supernatant. Optionally, add BSA to achieve a final concentration of 0.1% for extra blocking.
8. Add 200 μL of mag storage buffer to the tube for the 20 µg scale or 1 mL to each tube for the 200 µg scale, mix thoroughly, use a magnetic stand to collect the particles, and discard the supernatant.
9. Repeat the washing step twice.
10. Add 25 μL of mag storage buffer to the tube for the 20 µg scale or 125 μL to each tube for the 200 µg scale, mix thoroughly, and pool the tubes for the 200 µg scale.
11. The result is a 25 μL/250 μL 1% conjugate. Further dilute as needed for your application and consider adding a preservative if desired.
*To estimate the amount of antibody bound to the particles, analyze the supernatant using a Bradford protein assay and calculate the difference from the initial amount.
Please refer to the COA of the shipped product.
- Product Precautions
- This product is intended for research purposes only and is not intended for use in human, therapeutic, or diagnostic applications.
- Kit Storage
- Upon receipt, store the kit at -20°C immediately and refer to the storage instructions for individual components below. The kit can be stored for up to 1 year if components have not been reconstituted. It is important to avoid multiple freeze-thaw cycles of reagents.
- Conjugate Storage
- It is advisable to store any conjugate at 4°C. The mag storage buffer used during the conjugation process is an effective storage solution. Avoid storing the conjugate at -20°C.
- Sample Type
- Antibody sample
- Reactive Species
- The reactive species of the kit depends on the research application of the antibody.
- Fluorescent Dye Introduction
- Magnetic conjugation refers to the process of pairing magnetic particles or objects in such a way that they attract each other due to their magnetic properties. This can occur with magnets or other materials that have magnetic properties, such as iron or nickel.
In magnetic conjugation, the magnetic fields of the objects align in such a way that they are drawn together with a force that depends on the strength of the magnetic fields and the distance between the objects. This process is often used in various applications, such as in magnetically coupled pumps or magnetic levitation systems.
Overall, magnetic conjugation involves the manipulation of magnetic fields to bring objects together, allowing for the creation of unique and innovative solutions in various fields of technology.
- Alter Name
- Quick™ Magnetic Antibody Labeling Kit; Magnetic Antibody Labeling Kit
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS-0824-FY98. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
-1.png)
Application: ELISA, FC, Block, SPR
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FC, Inhib, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, FuncS, RIA, IP, IF, IHC, IA, Block
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
