Mouse Anti-ALCAM Recombinant Antibody (clone 8E12C7) (CAT#: VS3-XY41)
Clone 8E12C7 is a mouse antibody, which reacts with human ALCAM protein. This antibody is suitable for ALCAM detection by ELISA, WB, IHC, FC, ICC assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/52_B2_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/52_B2_2_selected.jpg
Figure 2 Parathyroid gland
(High expression in parathyroid gland)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_1_8_val_selected.jpg
(High expression in parathyroid gland)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_1_8_val_selected.jpg
Figure 3 Kidney
(Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_7_5.jpg
(Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_7_5.jpg
Figure 4 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_B_7_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_B_7_5.jpg
Figure 5 Stomach
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_6_1.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_6_1.jpg
Figure 6 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_9_3.jpg
Figure 7 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_9_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10926/26851_A_9_4.jpg
Figure 8 RNA cell line category: Cell line enhanced (hTERT-HME1, U-138 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170017-ALCAM
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170017-ALCAM
Specifications
- Immunogen
- Recombinant fragment of human ALCAM (a.a. 48-216) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human ALCAM
- Species Reactivity
- Human
- Clone
- 8E12C7
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).
Target
- Alternative Names
- Activated Leukocyte Cell Adhesion Molecule; CD166 Antigen; MEMD; Activated Leucocyte Cell Adhesion Molecule; CD166
- Gene ID
- 214
- UniProt ID
- Q13740
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-031CL | Human Anti-ALCAM Recombinant Antibody (TAB-031CL) | ELISA | Human IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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