Mouse Anti-B2M Recombinant Antibody (clone 4G5A1)
CAT#: VS3-XY109
Clone 4G5A1 is a mouse antibody, which reacts with human B2M protein. This antibody is suitable for B2M detection by ELISA, WB, IHC, FC, ICC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/if_selected.jpg
Normal Tissue
Figure 3 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6361/18752_B_7_4_val_selected.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_6_4.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_B_8_5.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2572/6948_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166710-B2M
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Specifications
- Immunogen
- Recombinant fragment of human B2M (a.a. 21-100) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human B2M
- Species Reactivity
- Human
- Clone
- 4G5A1
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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