Mouse Anti-CCL2 Recombinant Antibody (clone 1A7B8)
CAT#: VS3-XY177
Clone 1A7B8 is a mouse antibody, which reacts with human CCL2 protein. This antibody is suitable for CCL2 detection by ELISA, WB assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/1742_B4_8_cr580492c5c7769_selected.jpg
Subcellular Location
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in dot like pattern in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human appendix
Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_2_2_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/182_D1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_8_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (BJ hTERT+, HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
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Specifications
- Immunogen
- Recombinant fragment of human CCL2 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CCL2
- Species Reactivity
- Human
- Clone
- 1A7B8
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000) and Enzyme-linked Immunosorbent Assay (1/10000).
Target
- Alternative Names
- C-C Motif Chemokine Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE; Small Inducible Cytokine A2 (Monocyte Chemotactic Protein 1, Homologous To Mouse Sig-Je); Small Inducible Cytokine Subfamily A (Cys-Cys), Member 2; Monocyte Chemoattractant Protein-1; Chemokine (C-C Motif) Ligand 2; Monocyte Chemotactic Protein 1; Small-Inducible Cytokine A2; SCYA2; MCP-1
- Gene ID
- 6347
- UniProt ID
- P13500
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Related Diseases
Rheumatoid Arthritis
Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
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