Mouse Anti-CD14 Recombinant Antibody (clone 1H5D8) (CAT#: VS3-XY188)
Clone 1H5D8 is a mouse antibody, which reacts with human CD14 protein. This antibody is suitable for CD14 detection by ELISA, WB, IHC, ICC assays.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/1183_E12_3_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/1183_E12_3_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_B_8_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_B_8_5.jpg
Figure 3 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_7_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_7_3.jpg
Figure 4 Testis
(Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_4_6.jpg
(Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_4_6.jpg
Figure 5 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_9_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_9_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (A549, CACO-2, SiHa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170458-CD14
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170458-CD14
Figure 6 RNA cell line category: Cell line enhanced (A549, CACO-2, SiHa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170458-CD14
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170458-CD14
Specifications
- Immunogen
- Recombinant fragment of human CD14 (a.a. 20-214) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human CD14
- Species Reactivity
- Human
- Clone
- 1H5D8
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000) and Immunocytochemistry (1/200-1/1000).
Target
- Alternative Names
- CD14 Molecule; CD14 Antigen; Myeloid Cell-Specific Leucine-Rich Glycoprotein
- Gene ID
- 929
- UniProt ID
- P08571
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1583CL | Anti-Human CD14 Recombinant Antibody (18D11) | FC | |
| TAB-1584CL | Anti-Human CD14 Recombinant Antibody (Mil2) | FC, ELISA | |
| TAB-1587CL | Anti-Human CD14 Recombinant Antibody (ZCH-7-2F9) | ELISA, WB | |
| TAB-1583CL-S(P) | Anti-Human CD14 Recombinant Antibody scFv Fragment (18D11) | FC | |
| TAB-1584CL-S(P) | Anti-Human CD14 Recombinant Antibody scFv Fragment (Mil2) | FC, ELISA |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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