Mouse Anti-CD27 scFv-Fc Chimera (VS-0425-FY16)
CAT#: VS-0425-FY16
The anti-CD27 scFv-Fc is engineered to bind specifically to the CD27 receptor, which plays a critical role in T-cell activation and proliferation. By using an scFv-Fc format, this product achieves improved binding affinity and extended half-life in circulation, facilitating enhanced therapeutic performance. This monoclonal antibody fusion can be utilized in cancer treatments to promote T-cell responses or in immune modulation therapies to enhance immune system function. Its capacity to activate immune cells makes it a valuable tool for further research and clinical applications.
Gene Expression
Normal Tissue
Figure 1 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2420/6097_A_8_8.jpg
Normal Tissue
Figure 2 Tonsil
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2420/6097_A_5_8.jpg
Normal Tissue
Figure 3 High expression in lymph node.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_7_8_val_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_8_8.jpg
Normal Tissue
Figure 5 Tonsil
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_5_8.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (Daudi, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139193-CD27
RNA Expression
Figure 3 RNA cell line category: Group enriched (Daudi, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139193-CD27
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Specifications
- Immunogen
- The immunogen was a soluble human CD27-Fc fusion protein.
- Host Species
- Mouse
- Type
- Mouse IgG1, scFv-Fc
- Specificity
- The antibody binds to human CD27.
- Species Reactivity
- Human
- Applications
- Inhibition, ELISA, Flow Cytometry
- Related Disease
- Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- CD27 Molecule; Tumor Necrosis Factor Receptor Superfamily, Member 7; T-Cell Activation Antigen CD27; CD27L Receptor; CD27 Antigen; TNFRSF7; T14
- Gene ID
- 939
- UniProt ID
- P26842
- Long Name
- CD27 Molecule
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Phosphorylated.
N-glycosylated.
O-glycosylated with core 1 or possibly core 8 glycans.
Glycosylation at Asn95 and Ser127
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect CD27_HUMAN (800)
Glycosylation from GlyGen 4 sites, 3 N-linked glycans (1 site), 2 O-linked glycans (3 sites)
- Protein Refseq
- XP_011519344.1; XP_016875723.1; XP_047285856.1; XP_054229842.1; XP_054229843.1
- Function
- Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells.
The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells.
Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory.
In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis.
Negatively regulates the function of Th2 lymphocytes in the adipose tissue.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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