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    Mouse Anti-CHD3 Recombinant Antibody (clone 5B2E5) (CAT#: VS3-XY497)

    Clone 5B2E5 is a mouse antibody, which reacts with human CHD3 protein. This antibody is suitable for CHD3 detection by ELISA, WB, IHC, FC, ICC assays.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1674_H9_32_selected.jpg
    Figure 2 IHC staining of human cerebral cortex
    Figure 2 IHC staining of human cerebral cortex
    (Immunohistochemical staining of human cerebral cortex shows distinct nuclear positivity in neuronal cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/ihc_selected.jpg
    Figure 3 IF staining of human cell line REH
    Figure 3 IF staining of human cell line REH
    (Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & nucleoli.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1676_H9_2_red_green.jpg
    Figure 4 IF staining of human cell line SiHa
    Figure 4 IF staining of human cell line SiHa
    (Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & nucleoli.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/76524/1712_C4_3_cr5804e5a4d2793_red_green.jpg
    Normal Tissue
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_B_8_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endocrine cells
    Staining:Medium
    Intensity: Moderate
    Endothelial cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Enterocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Fibroblasts
    Staining:High
    Intensity: Strong
    Mucosal lymphoid cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Peripheral nerve/ganglion
    Staining:High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_7_3.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Sertoli cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43368/118517_A_6_6.jpg
    RNA Expression
    Figure 8 RNA cell line category: Low cell line specificity
    Figure 8 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170004-CHD3

    Specifications

    • Immunogen
    • Recombinant fragment of human CHD3 expressed in E. coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human CHD3
    • Species Reactivity
    • Human
    • Clone
    • 5B2E5
    • Applications
    • Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry

    Product Property

    • Purification
    • Affinity purified
    • Format
    • Liquid
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).

    Target

    • Alternative Names
    • ZFH; Mi-2a; SNIBCPS; Mi2-ALPHA

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "CHD3"

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    CAT Product Name Application Type
    MOR-0685 Hi-Affi™ Rabbit Anti-CHD3 Recombinant Antibody (clone DS685AB) WB, IHC, ICC Rabbit IgG

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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