Mouse Anti-DKK3 Recombinant Antibody (clone 8A5C6) (CAT#: VS3-XY580)
Clone 8A5C6 is a mouse antibody, which reacts with human DKK3 protein. This antibody is suitable for DKK3 detection by ELISA, WB, IHC, FC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/89_C8_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/89_C8_2_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_B_8_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_3.jpg
Figure 4 Liver
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_4.jpg
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_5_6.jpg
Figure 7 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-RPE1, TIME)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000050165-DKK3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000050165-DKK3
Specifications
- Immunogen
- Recombinant fragment of human DKK3 (a.a. 91-350) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human DKK3
- Species Reactivity
- Human
- Clone
- 8A5C6
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Immunohistochemistry(1/200-1/1000).
Target
- Alternative Names
- Dickkopf WNT Signaling Pathway Inhibitor 3; Regulated In Glioma; Dickkopf-3; HDkk-3; Dkk-3; REIC; Dickkopf (Xenopus Laevis) Homolog 3; Dickkopf 3 Homolog (Xenopus Laevis)
- Gene ID
- 27122
- UniProt ID
- Q9UBP4
Related Resources
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0055CT | Recombinant Mouse anti-Human DKK3 Monoclonal antibody (5H8) | ELISA, FC, WB | |
| HPAB-2833LY | Mouse Anti-DKK3 Recombinant Antibody (HPAB-2833LY) | ELISA, FC, Block | Mouse IgG |
| VS3-QX326 | Mouse Anti-DKK3 Recombinant Antibody (clone 4G7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-HM818 | Mouse Anti-DKK3 Recombinant Antibody (clone CBL197HM) | WB, FCM, ELISA | Mouse IgG |
| VS13-YC304 | Rabbit Anti-DKK3 Recombinant Antibody (VS13-YC304) | WB, ICC, IF, IP | Rabbit IgG |
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