Mouse Anti-ERCC1 Recombinant Antibody (clone 1H10D2)
CAT#: VS3-XY648
Clone 1H10D2 is a mouse antibody, which reacts with human ERCC1 protein. This antibody is suitable for ERCC1 detection by ELISA, WB, ICC, FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50182/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72859/157426_A_4_1_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_9_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_5_6.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_B_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_A_8_8.jpg
Normal Tissue
Figure 7 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72860/157430_B_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000012061-ERCC1
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Specifications
- Immunogen
- Recombinant fragment of human ERCC1 (a.a. 151-297) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ERCC1
- Species Reactivity
- Human
- Clone
- 1H10D2
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunocytochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).
Target
- Alternative Names
- ERCC Excision Repair 1, Endonuclease Non-Catalytic Subunit; Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 1 (Includes Overlapping Antisense Sequence); Excision Repair Cross-Complementation Group 1; DNA Excision Repair Protein ERCC-1; COFS4; RAD10; UV20
- Gene ID
- 2067
- UniProt ID
- P07992
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ERCC1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1931z | Mouse Anti-ERCC1 Recombinant Antibody (clone 21G8) | WB, ICC, IF, IHC | Mouse IgG1 |
| MRO-0541-CN | Rabbit Anti-ERCC1 Recombinant Antibody (clone JM10-07) | WB, IF, IHC | Rabbit IgG |
| ZG-0314J | Mouse Anti-ERCC1 Recombinant Antibody (ZG-0314J) | WB | Mouse IgG |
| VS3-XY649 | Mouse Anti-ERCC1 Recombinant Antibody (clone 9D10B7) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS4-WK165 | Mouse Anti-ERCC1 Recombinant Antibody (VS4-WK165) | ELISA, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1183 | Hi-Affi™ Rabbit Anti-ERCC1 Recombinant Antibody (clone DS1183AB) | FC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2341 | Anti-ERCC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2342 | Anti-Mouse ERCC1 Immunohistochemistry Kit | IHC |
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