Mouse Anti-GAPDH (clone W25lesion) scFv-Fc Chimera
CAT#: VS-0125-FY39
Anti-GAPDH scFv-Fc is a fusion protein composed of a single-chain antibody (scFv) portion and an immunoglobulin Fc portion, specifically targeting glyceraldehyde-3-phosphate dehydrogenase (GAPDH), an enzyme that is widely present in cells. GAPDH plays a crucial role in glycolysis and other cellular processes, making the regulation of its expression and function significant for studying cellular metabolism and pathological processes.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/752_B3_1_selected.jpg
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1, scFv-Fc
- Species Reactivity
- Human
- Clone
- W25lesion
- Applications
- ELISA, Western Blot
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Glyceraldehyde-3-Phosphate Dehydrogenase; Peptidyl-Cysteine S-Nitrosylase GAPDH; EC 1.2.1.12; GAPD; Epididymis Secretory Sperm Binding Protein Li 162eP; Oct1 Coactivator In S Phase, 38 Kd Component; Aging-Associated Gene 9 Protein
- Gene ID
- 2597
- UniProt ID
- P04406
- Sequence Similarities
- Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
- Cellular Localization
- Cytoplasm, cytosol
Nucleus
Cytoplasm, perinuclear region
Membrane
Cytoplasm, cytoskeleton
- Post Translation Modifications
- S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity).
S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.
ISGylated.
Sulfhydration at Cys-152 increases catalytic activity.
Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation.
- Protein Refseq
- NP_001243728.1; NP_001276674.1; NP_001276675.1; NP_001344872.1; NP_002037.2
- Function
- Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Modulates the organization and assembly of the cytoskeleton (By similarity).
Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0125-FY39. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
See other products for "GAPDH"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-386-F(E) | Recombinant Anti-human GAPDH Antibody Fab Fragment | IP, RIA, FuncS | Fab |
| MHH-386-F(E) | Recombinant Human Anti-human GAPDH Antibody Fab Fragment | ELISA, WB, IP, FuncS | Fab |
| FAMAB-0117-YC-F(E) | Human Anti-GAPDH Recombinant Antibody (clone lesion); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-386-S(P) | Recombinant Human Anti-human GAPDH Antibody scFv Fragment | WB, FC, FuncS | scFv |
| FAMAB-0117-YC-S(P) | Human Anti-GAPDH Recombinant Antibody (clone lesion); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0217MZ | Chicken Anti-GAPDH Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1398 | Hi-Affi™ Rabbit Anti-GAPDH Recombinant Antibody (clone DS1398AB) | WB, ICC, IP, FC | Rabbit IgG |
| MOR-0101-FY | Rabbit Anti-GAPDH Recombinant Antibody (clone AFY0072) | IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0117YC | Human Anti-GAPDH Recombinant Antibody (clone lesion) | ELISA, WB | Human IgG |
| ZG-0290U | Mouse Anti-GAPDH Recombinant Antibody (clone 14C2F11) | ELISA, WB, IHC, IP, IF, FC | Mouse IgG1 |
| ZG-0291U | Mouse Anti-GAPDH Recombinant Antibody (clone 10B4E3) | ELISA, WB, IHC, IP, IF | Mouse IgG1 |
| ZG-0292U | Mouse Anti-GAPDH Recombinant Antibody (clone 10H4D6) | ELISA, WB, IHC, IP, IF | Mouse IgG1 |
| VS3-FY2715 | Mouse Anti-GAPDH Recombinant Antibody (VS3-FY2715) | WB, IHC, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0632-CN | Mouse Anti-GAPDH Recombinant Antibody (clone 5-E10) | WB, IF | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY108 | AbPlus™ Anti-GAPDH Magnetic Beads (VS-0424-XY108) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY212 | Mouse Anti-NHP GAPDH Recombinant Antibody (clone 1D4) | WB, IF | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1224-YC534 | DrugMonitor™ Anti-GAPDH Antibody (VS-1224-YC534) | ELISA, PK/PD, ADA control | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY896 | Anti-GAPDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY2729 | Anti-Mouse GAPDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY2728 | Anti-Human GAPDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY2730 | Anti-Goat GAPDH Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC435 | CytoStream™ Rabbit Anti-GAPDH Recombinant Antibody (VS13-YC435) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA
Application: ELISA, WB, FC, IHC, IP
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
-2.png)
Application: IB, ELISA, FC, FuncS
Application: ELISA, FuncS
Application: ELISA, WB, FC, IHC, IP
Application: ELISA
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
