Mouse Anti-IL12B scFv-Fc Chimera (VS-0425-FY122) (CAT#: VS-0425-FY122)

• Immunogen
• The immunogen was recombinant purified human IL-12 p40.

• Host Species
• Mouse

• Type
• Mouse IgG1, scFv-Fc

• Specificity
• Human IL12B

• Species Reactivity
• Human, Cynomolgus monkey

• Applications
• Inhibition

• Related Disease
• Acute and chronic inflammatory disease

• Purity
• >95% as determined by SDS-PAGE

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• The potency of the purifed monoclonal antibody in inhibiting interleukin-12 (IL-12) activity was assessed using the phytohaemagglutinin (PHA) blast assay. The half-maximal inhibit concentration (IC₅₀) values derived from this assay indicated that human IL-12 was inhibited at an IC₅₀ of approximately 0.31 nM, while the corresponding value for cynomolgus monkey IL-12 was approximately 1.4 nM.
  The optimal dilution concentration is determined by the experiment.

• Alternative Names
• Interleukin 12B

• Gene ID
• 3593

• UniProt ID
• P29460

• Long Name
• Interleukin 12B

• Sequence Similarities
• Belongs to the IL-12B family.

• Cellular Localization
• Secreted

• Post Translation Modifications
• Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified.
  Glycosylation at Asn135, Asn222, and Trp319
  Modification sites at PhosphoSitePlus
  Glycosylation from GlyGen 3 sites, 1 C-linked glycan (1 site)

• Protein Refseq
• NP_002178.2

• Function
• Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. IL12B_HUMAN,P29460
  Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity.
  IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues.
  IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines.
  IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

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