Mouse Anti-ILK Recombinant Antibody (clone 3A9)
CAT#: VS3-XY879
Clone 3A9 is a mouse antibody, which reacts with ILK protein. This antibody is suitable for ILK detection by ELISA, WB, IHC, FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48437/728_B5_2_selected.jpg
Normal Tissue
Figure 2 Seminal vesicle.
High expression in seminal vesicle
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4041/11596_A_9_6_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4041/11596_B_9_5.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4041/11596_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4041/11596_A_5_6.jpg
Normal Tissue
Figure 6 Smooth muscle
Smooth muscle cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4041/11596_B_6_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4041/11596_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166333-ILK
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Specifications
- Immunogen
- Recombinant fragment of human ILK (a.a. 97-244) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- ILK
- Species Reactivity
- Human
- Clone
- 3A9
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Immunohistochemistry(1/200-1/1000).
Target
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Related Diseases
Endometrial Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 3A9"
- CAT
- Product Name
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2028MZ | Recombinant Mouse Anti-Human ILK Antibody | WB | Mouse antibody |
| MRO-0857-CN | Rabbit Anti-ILK Recombinant Antibody (clone CBACN-317) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0767J | Rabbit Anti-ILK Recombinant Antibody (clone 6A5) | ELISA, WB, IF, IP | Rabbit IgG |
| VS3-WK931 | Rabbit Anti-ILK Recombinant Antibody (VS3-WK931) | WB, ICC, IHC, IP, FC | Rabbit IgG |
| VS3-FY755 | Recombinant Rabbit Anti-ILK Antibody (clone R01-7F8) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1821 | Rabbit Anti-ILK Recombinant Antibody (clone DS1821AB) | WB, FC, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY280 | Mouse Anti-NHP ILK Recombinant Antibody (clone 3A9) | WB, FC, ELISA | Mouse IgG1 |
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