Mouse Anti-KEAP1 Recombinant Antibody (clone 1F10B6) (CAT#: VS3-XY966)
Clone 1F10B6 is a mouse antibody, which reacts with human KEAP1 protein. This antibody is suitable for KEAP1 detection by ELISA, WB, ICC, FC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & centriolar satellites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5558/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & centriolar satellites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5558/if_selected.jpg
Figure 2 Cerebral cortex
(Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_B_9_5.jpg
(Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_B_9_5.jpg
Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_9_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_9_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5558/15846_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5558/15846_A_9_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/25337/55768_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079999-KEAP1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079999-KEAP1
Specifications
- Immunogen
- Recombinant fragment of human KEAP1 (a.a. 380-624) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KEAP1
- Species Reactivity
- Human
- Clone
- 1F10B6
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunocytochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).
Target
- Alternative Names
- Kelch Like ECH Associated Protein 1; Kelch-Like Family Member 19; Cytosolic Inhibitor Of Nrf2; Kelch-Like Protein 19; KLHL19; INrf2; Kelch-Like ECH-Associated Protein 1; KIAA0132
- Gene ID
- 9817
- UniProt ID
- Q14145
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4068z | Mouse Anti-KEAP1 Recombinant Antibody (clone 22D2) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| VS3-XY967 | Mouse Anti-KEAP1 Recombinant Antibody (clone 7G4B10) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS3-FY2749 | Rabbit Anti-KEAP1 Recombinant Antibody (VS3-FY2749) | WB, IHC | Rabbit IgG |
| VS13-YC618 | Rabbit Anti-KEAP1 Recombinant Antibody (VS13-YC618) | WB, IHC | Rabbit IgG |
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