Mouse Anti-MCAM Recombinant Antibody (clone 6C3E6) (CAT#: VS3-XY1054)
Clone 6C3E6 is a mouse antibody, which reacts with MCAM protein. This antibody is suitable for MCAM detection by ELISA, WB, IHC, FC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2147/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2147/if_selected.jpg
Figure 2 Soft tissue
(Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_2_7.jpg
(Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_2_7.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_9_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_9_5.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_8_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_8_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_7_5.jpg
Figure 6 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Weak Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_4_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Weak Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_4_6.jpg
Figure 7 RNA cell line category: Group enriched (HUVEC TERT2, TIME, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076706-MCAM
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076706-MCAM
Specifications
- Immunogen
- Recombinant fragment of human MCAM (a.a. 84-189) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MCAM
- Species Reactivity
- Human
- Clone
- 6C3E6
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Immunohistochemistry(1/200-1/1000).
Target
- Alternative Names
- Melanoma Cell Adhesion Molecule; S-Endo 1 Endothelial-Associated Antigen; Melanoma-Associated Antigen MUC18; Cell Surface Glycoprotein P1H12; Melanoma-Associated Antigen A32; Gicerin
- Gene ID
- 4162
- UniProt ID
- P43121
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-516CL | Anti-Human MCAM Recombinant Antibody (ABX-MA1) | WB, FuncS | Antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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