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Mouse Anti-NES Recombinant Antibody (clone 3H12C8) (CAT#: VS3-XY1155)

Clone 3H12C8 is a mouse antibody, which reacts with human NES protein. This antibody is suitable for NES detection by ELISA, WB, FC assays.


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Subcellular Location
Figure 1 IF staining of human cell line RH-30
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6286/1401_E3_2_selected.jpg
Figure 2 IF staining of human cell line AF22
Figure 2 IF staining of human cell line AF22
(Immunofluorescent staining of human cell line AF22 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/1783_C4_5_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5889/613_B1_1_selected.jpg
Normal Tissue
Figure 4 Spleen
Figure 4 Spleen
(High expression in spleen)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7007/20723_B_8_4_val_selected.jpg
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_7_5.jpg
Figure 6 Colon
Figure 6 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_7_3.jpg
Figure 7 Kidney
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_8_5.jpg
Figure 8 Heart muscle
Figure 8 Heart muscle
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (AF22, HUVEC TERT2, LHCN-M2, RH-30, SuSa, WM-115)
Figure 9 RNA cell line category: Cell line enhanced (AF22, HUVEC TERT2, LHCN-M2, RH-30, SuSa, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132688-NES

Specifications

  • Immunogen
  • Recombinant fragment of human NES (a.a. 419-588) expressed in E. coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human NES
  • Species Reactivity
  • Human
  • Clone
  • 3H12C8
  • Applications
  • Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry

Product Property

  • Format
  • Liquid
  • Preservative
  • 0.03% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Flow Cytometry (1/200-1/400).

Target

  • Alternative Names
  • Nestin

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Chicken IgY Antibody

CAT Product Name Application Type
BRD-0721MZ Chicken Anti-Nes Polyclonal IgY ICC/IF, IHC, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2422 Hi-Affi™ Recombinant Rabbit Anti-NES Monoclonal Antibody (DS2422AB) IHC-P, WB IgG
MOR-0108-FY Rabbit Anti-NES Recombinant Antibody (clone AFY0079) ICC, IHC, WB Rabbit IgG

Recombinant Antibody

CAT Product Name Application Type
MRO-2064-CN Rabbit Anti-NES Polyclonal Antibody (MRO-2064-CN) IHC Rabbit IgG
MRO-2065-CN Rabbit Anti-NES Polyclonal Antibody (MRO-2065-CN) IF, IHC, FC, WB Rabbit IgG
VS1-YJ144 Mouse Anti-NES Recombinant Antibody (clone 10C2), FITC ELISA, IF, IHC-Fr, IHC-P, WB, FC, IHC Mouse IgG1
VS1-YJ145 Mouse Anti-NES Recombinant Antibody (clone 10C2), HRP ELISA, IF, IHC-Fr, IHC-P, WB, FC, IHC Mouse IgG1
VS3-XY1154 Mouse Anti-NES Recombinant Antibody (clone 3H12B9) ELISA, WB, FC Mouse IgG1

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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