Mouse Anti-USP7 Recombinant Antibody (clone 5D5B8) (CAT#: VS3-XY1581)
Clone 5D5B8 is a mouse antibody, which reacts with USP7 protein. This antibody is suitable for USP7 detection by ELISA, WB assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79904/1987_C9_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79904/1987_C9_4_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/ihc_selected.jpg
(Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/ihc_selected.jpg
Figure 3 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/35011_B_7_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/35011_B_7_5.jpg
Figure 4 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/35011_A_8_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/35011_A_8_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/35011_A_9_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/35011_A_9_4.jpg
Figure 6 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/35011_A_5_6.jpg
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/35011_A_5_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/35011_A_7_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15641/35011_A_7_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000187555-USP7
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000187555-USP7
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000187555-USP7
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000187555-USP7
Specifications
- Immunogen
- Recombinant fragment of human USP7 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- USP7
- Species Reactivity
- Human
- Clone
- 5D5B8
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000) and Enzyme-linked Immunosorbent Assay (1/10000).
Target
- Alternative Names
- Ubiquitin Specific Peptidase 7; Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated); Ubiquitin-Specific-Processing Protease 7; Deubiquitinating Enzyme 7; Ubiquitin Thioesterase 7; HAUSP; Ubiquitin Specific Protease 7 (Herpes Virus-Associated)
- Gene ID
- 7874
- UniProt ID
- Q93009
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-641 | Human Anti-USP7 Recombinant Antibody (clone D545) | ELISA, IP-MS | Human IgG |
| MRO-1564-CN | Rabbit Anti-USP7 Recombinant Antibody (clone JB80-36) | WB, IHC, FC | Rabbit IgG |
| ZG-0407J | Mouse Anti-USP7 Recombinant Antibody (ZG-0407J) | WB, ELISA | Mouse IgG |
| ZG-0408J | Mouse Anti-USP7 Recombinant Antibody (ZG-0408J) | WB, IF | Mouse IgG |
| VS3-QX1226 | Mouse Anti-USP7 Recombinant Antibody (clone 3E4-D10-E8) | WB | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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