Mouse Anti-VIM (clone HoAKs-1) scFv-Fc Chimera (CAT#: VS-0425-FY193)

• Immunogen
• The immunogen used to generate the HoAKs-1 antibody was the approximately 55 kDa protein (vimentin) derived from the human pancreatic cancer cell line PANC-1.

• Host Species
• Mouse

• Type
• Mouse IgG1, scFv-Fc

• Specificity
• Human VIM

• Species Reactivity
• Human

• Clone
• HoAKs-1

• Applications
• Western Blot, Immunohistochemistry, Flow Cytometry

• Related Disease
• Cataract 30, Multiple Types and Cataract 30

• Purity
• >95% as determined by SDS-PAGE

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500-1:5000
  Immunohistochemistry: 1:100-1:1000
  ELISA: 1:100-1:10000
  The optimal dilution concentration is determined by the experiment.

• Alternative Names
• Vimentin

• Gene ID
• 7431

• UniProt ID
• P08670

• Long Name
• Vimentin

• Sequence Similarities
• Belongs to the intermediate filament family.

• Cellular Localization
• Cytoplasm; Cytoplasm, cytoskeleton; Nucleus matrix; Cell membrane

• Post Translation Modifications
• O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
  S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  Glycosylation at Ser7, Thr33, and Ser34
  Ubiquitination at Lys97, Lys120, Lys129, Lys139, Lys143, Lys168, Lys188, Lys223, Lys236, Lys282, Lys294, Lys313, Lys334, Lys373, Lys439, and Lys445
  Modification sites at PhosphoSitePlus
  Glycosylation from GlyConnect VIME_HUMAN (2867)
  Glycosylation from GlyGen 26 sites, 2 N-linked glycans (2 sites), 2 O-linked glycans (24 sites)

• Protein Refseq
• NP_003371.2

• Function
• Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
  Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
  Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front.
  Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner.

• TAB-353CQ
• Anti-Human VIM Recombinant Antibody (HoAKs-1)

• TAB-353CQ-S(P)
• Anti-Human VIM Recombinant Antibody scFv Fragment (HoAKs-1)

• TAB-353CQ-F(E)
• Anti-Human VIM Recombinant Antibody Fab Fragment (HoAKs-1)

• TAB-353CQ-mRNA
• Mouse Anti-VIM Antibody (clone HoAKs-1), mRNA

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