Mouse Anti-VIM (clone HoAKs-1) scFv-Fc Chimera
CAT#: VS-0425-FY193
The anti-VIM scFv-Fc is a targeted molecule designed to bind vimentin, potentially disrupting its functions or leading to the elimination of vimentin-expressing cells or the neutralization of extracellular vimentin. Its applications could be relevant in various diseases characterized by vimentin upregulation, such as cancer, fibrosis, and inflammatory conditions. The specific application and mechanism of action will depend on the chosen scFv's specificity for different forms or locations of vimentin and the effector functions of the Fc region.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:
High
Intensity: Strong
Mucosal lymphoid cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:
High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg
Normal Tissue
Figure 8 Breast
Adipocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Myoepithelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM
❮
❯
Specifications
- Immunogen
- The immunogen used to generate the HoAKs-1 antibody was the approximately 55 kDa protein (vimentin) derived from the human pancreatic cancer cell line PANC-1.
- Host Species
- Mouse
- Type
- Mouse IgG1, scFv-Fc
- Specificity
- Human VIM
- Species Reactivity
- Human
- Clone
- HoAKs-1
- Applications
- Western Blot, Immunohistochemistry, Flow Cytometry
- Related Disease
- Cataract 30, Multiple Types and Cataract 30
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500-1:5000
Immunohistochemistry: 1:100-1:1000
ELISA: 1:100-1:10000
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Vimentin
- Gene ID
- 7431
- UniProt ID
- P08670
- Long Name
- Vimentin
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm; Cytoplasm, cytoskeleton; Nucleus matrix; Cell membrane
- Post Translation Modifications
- O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Glycosylation at Ser7, Thr33, and Ser34
Ubiquitination at Lys97, Lys120, Lys129, Lys139, Lys143, Lys168, Lys188, Lys223, Lys236, Lys282, Lys294, Lys313, Lys334, Lys373, Lys439, and Lys445
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect VIME_HUMAN (2867)
Glycosylation from GlyGen 26 sites, 2 N-linked glycans (2 sites), 2 O-linked glycans (24 sites)
- Protein Refseq
- NP_003371.2
- Function
- Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front.
Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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- CAT
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-353CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (HoAKs-1) | ELISA, WB | |
| TAB-354CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (2F6-1) | ELISA, WB | |
| TAB-355CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (3F9-1) | ELISA, WB | |
| TAB-353CQ-F(E) | Anti-Human VIM Recombinant Antibody Fab Fragment (HoAKs-1) | ELISA, WB | |
| TAB-354CQ-F(E) | Anti-Human VIM Recombinant Antibody Fab Fragment (2F6-1) | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0741MZ | Chicken Anti-VIM Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3838 | Rabbit Anti-VIM Recombinant Antibody (clone DS3838AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1583-CN | Mouse Anti-VIM Recombinant Antibody (clone A6-C1) | WB, IF, IHC, FC | Mouse IgG2b |
| MRO-1584-CN | Rabbit Anti-VIM Recombinant Antibody (clone CBACN-580) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-1585-CN | Mouse Anti-VIM Recombinant Antibody (clone D4-B11) | WB, IF, IHC, FC | Mouse IgG1 |
| ZG-0499F | Mouse Anti-VIM Recombinant Antibody (ZG-0499F) | WB, IHC, ELISA | Mouse IgG |
| ZG-0500F | Mouse Anti-VIM Recombinant Antibody (ZG-0500F) | WB, IHC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2717LY-F(E) | Human Anti-VIM Recombinant Antibody Fab Fragment (HPAB-2717LY-F(E)) | FC, WB, Block, ELISA | Humanized Fab |
| HPAB-0349-YJ-F(E) | Mouse Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0349-YJ-F(E)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse Fab |
| HPAB-0350-YJ-F(E) | Human Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0350-YJ-F(E)) | ELISA, WB, IP, ICC, IHC, FC, IF | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2717LY-S(P) | Human Anti-VIM Recombinant Antibody scFv Fragment (HPAB-2717LY-S(P)) | FC, WB, Block, ELISA | Humanized scfv |
| HPAB-0349-YJ-S(P) | Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0349-YJ-S(P)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse scFv |
| HPAB-0350-YJ-S(P) | Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0350-YJ-S(P)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF038 | DRB1*04:01/Human cit vimentin (GVYAT[cit]SSAV[cit]L[cit]SSVPGVR) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1237 | Mouse Anti-VIM Recombinant Antibody (clone 280CT3.4.6) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
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| VS-0424-XY274 | AbPlus™ Anti-VIM Magnetic Beads (NKI-M9) | IP, Protein Purification |
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