Protein G Agarose (VS-0824-WK12) (CAT#: VS-0824-WK12)
This protein G agarose is a premium-quality affinity resin for antibody purification. It consists of recombinant Protein G that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This particular variety of the affinity resin provides the most versatile combination of chromatographic features for high yield and high-purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.
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Specifications
- Product Size
- 2 mL
- Format
- Bottle
- Contents
- 2 mL settled agarose resin
- Specifications
- Support: crosslinked 6 % beaded agarose supplied as a 50 % slurry containing 0.02 % sodium azide
Immobilized phase: Protein G
Purification target: IgG antibodies
Binding capacity: 11-15 mg human IgG per mL of settled resin
- Storage
- Upon receipt store kit at 4°C.
- Background
- Protein G is a bacterial cell wall protein original from group G Streptococcus and now produced as a recombinant in E. coli. Like Protein A, Protein G binds to most mammalian immunoglobulins primarily through their Fc regions. Native Protein G contains two immunoglobulin binding sites, as well as albumin and cell surface binding sites. In the recombinant form of Protein G, these albumin and cell surface binding sites have been eliminated to reduce nonspecific binding when purifying immunoglobulins. Recombinant Protein G has a mass of approximately 21.6kDa, but its apparent size by SDS-PAGE is 31 to 34kDa. IgG-binding function is optimal at pH 5 but also occurs efficiently in near-neutral conditions (pH 7.0 to 7.2). Protein G Agarose is effective for affinity purification of IgG from serum and other fluids of many mammalian species. Protein G is especially well suited for use with mouse antibodies (including IgG1) in addition to most IgG isotypes from human, goat and sheep samples.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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