Rabbit Anti-ATP2A1 Polyclonal Antibody (MRO-1667-CN)
CAT#: MRO-1667-CN
This antibody is a rabbit polyclonal antibody that is capable of binding to ATP2A1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2310/if_selected.jpg
Normal Tissue
Figure 2 High expression in skeletal muscle.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32706/ihc_selected.jpg
Normal Tissue
Figure 3 Low expression in heart muscle.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32706/128663_B_5_6_val_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32706/128663_A_9_5.jpg
Normal Tissue
Figure 5 Skeletal muscle
Myocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32706/128663_B_1_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196296-ATP2A1
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Specifications
- Immunogen
- Synthetis peptide within rat ATP2A1 aa 20-50.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATP2A1 (ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 1)
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Peptide affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 0.5% BSA, 50% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 1; Calcium Pump 1; Calcium-Transporting ATPase Sarcoplasmic Reticulum Type, Fast Twitch Skeletal Muscle Isoform; ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1; Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 1; Endoplasmic Reticulum Class 1/2 Ca(2+) ATPase;
- Gene ID
- 487
- UniProt ID
- O14983
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Broad Species Reactivity
This polyclonal antibody is great because it reacts with human, mouse, and rat ATP2A1. We used it for Western Blotting of muscle lysates from different species, and it worked consistently well. It is a reliable tool for studying sarcoplasmic reticulum calcium pumps.
Good IHC Staining
We used this antibody for Immunohistochemistry on skeletal muscle sections. The staining pattern was consistent with the location of the sarcoplasmic reticulum. The peptide affinity purification seems to have resulted in a specific antibody with minimal background staining.
Q&As
-
What species does this antibody react with?
A: This Rabbit Anti-ATP2A1 Polyclonal Antibody is capable of binding to ATP2A1 from Human, Mouse, and Rat samples. It was raised against a synthetic peptide within rat ATP2A1.
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Can this antibody be used for Immunohistochemistry?
A: Yes, this antibody is validated for Immunohistochemistry (IHC), Western Blot (WB), and Flow Cytometry (FC). It detects the Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 1.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Signaling Pathways
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ATP2A1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2405CT | Mouse Anti-ATP2A1 Recombinant Antibody (clone 7B2) | WB, ELISA, IHC-P, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-032CQ | Mouse Anti-ATP2A1 Recombinant Antibody (clone IIH11) | Block, ELISA, IF, IHC, IHC-Fr, IP, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-033CQ | Mouse Anti-ATP2A1 Recombinant Antibody (clone VE121G9) | Block, ELISA, IF, IHC, IHC-Fr, IHC-P, IP, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0294 | Hi-Affi™ Rabbit Anti-ATP2A1 Recombinant Antibody (clone DS294AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS3-WK171 | Mouse Anti-ATP2A1 Recombinant Antibody (clone H6-D1) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS3-WK368 | Rabbit Anti-ATP2A1 Recombinant Antibody (clone JE45-75) | WB, IP, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-HM170 | Mouse Anti-ATP2A1 Recombinant Antibody (clone 4F11F6) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS7-HM171 | Mouse Anti-ATP2A1 Recombinant Antibody (clone 3D1H6) | WB, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY611 | Anti-ATP2A1 Immunohistochemistry Kit | IHC |
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