Rabbit Anti-FAP Polyclonal Antibody (MRO-1853-CN)
CAT#: MRO-1853-CN
This antibody is a rabbit polyclonal antibody that is capable of binding to FAP.
Gene Expression
Normal Tissue
Figure 1 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_9_3.jpg
Normal Tissue
Figure 2 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_8_4.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_7_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_4_6.jpg
Normal Tissue
Figure 5 Smooth muscle
Smooth muscle cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_B_6_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ hTERT+, hTERT-RPE1, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000078098-FAP
❮
❯
Specifications
- Immunogen
- Peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FAP (Fibroblast Activation Protein Alpha)
- Species Reactivity
- Human, Mouse
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Peptide affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 0.5% BSA, 50% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- Fibroblast Activation Protein Alpha; Seprase; 170 KDa Melanoma Membrane-Bound Gelatinase; Gelatine Degradation Protease FAP; Integral Membrane Serine Protease; Post-Proline Cleaving Enzyme; Surface-Expressed Protease; Dipeptidyl Peptidase FAP; FAPalpha; SIMP;
- Gene ID
- 2191
- UniProt ID
- Q12884
REVIEWS AND Q&AS
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Reliable for Fibroblast Studies
This polyclonal antibody has proven itself reliable in our fibroblast activation studies. Its high sensitivity in detecting FAP has greatly advanced our understanding of fibroblast roles in the tumor microenvironment, making it a valuable tool for our laboratory.
Strong Performance in Immunofluorescence
When employed in immunofluorescence protocols, this antibody consistently delivers high-quality images with minimal background, enhancing our ability to study FAP localization and function in various cellular contexts.
Dependable in Flow Cytometry
The polyclonal nature offers the versatility required for flow cytometry, enabling us to sort and quantify cells based on FAP expression with accuracy and reliability in each run.
Q&As
-
In which research applications is this antibody commonly used?
A: This antibody is typically utilized in Western blot (WB), immunofluorescence (IF), and flow cytometry (FC) to study the presence and role of FAP in various biological processes.
-
What are the advantages of using a polyclonal antibody like this one?
A: Polyclonal antibodies provide broader target recognition and enhanced sensitivity, which can be beneficial for detecting proteins with multiple epitopes or when working in variable conditions.
-
What kind of research benefits from studying FAP expression?
A: Research into cancer biology, fibrosis, and chronic inflammatory diseases can benefit from understanding FAP expression and activity, as it may reveal novel therapeutic targets.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Fap"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-211 | Anti-Human FAP Recombinant Antibody (TAB-211) | ELISA, IP, FC, FuncS, Neut, IF, WB | IgG1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-022WM | Human Anti-FAP Recombinant Antibody (TAB-022WM) | FC, ELISA | Human IgG1 |
| TAB-022WM-S(P) | Human Anti-FAP Recombinant Antibody; scFv Fragment (TAB-022WM-S(P)) | FC, ELISA | Human scFv |
| TAB-024WM-S(P) | Human Anti-FAP Recombinant Antibody; scFv Fragment (TAB-024WM-S(P)) | ELISA, FC, IF | Human scFv |
| TAB-022WM-F(E) | Human Anti-FAP Recombinant Antibody; Fab Fragment (TAB-022WM-F(E)) | FC, ELISA | Human Fab |
| TAB-024WM-F(E) | Human Anti-FAP Recombinant Antibody; Fab Fragment (TAB-024WM-F(E)) | ELISA, FC, IF | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-150LC-1 | Recombinant Anti-Human FAP Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
| Gly-151LC-1 | Recombinant Anti-Human FAP Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
| Gly-152LC-1 | Recombinant Anti-Human FAP Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
| Gly-153LC-1 | Recombinant Anti-Human FAP Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-153LC | Recombinant Anti-Human FAP Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0170-CN | Human Anti-FAP Recombinant Antibody (HPAB-0170-CN) | ELISA, IF | Human IgG |
| HPAB-0171-CN | Mouse Anti-FAP Recombinant Antibody (HPAB-0171-CN) | ELISA | Mouse IgG2a |
| HPAB-0173-CN | Human Anti-FAP Recombinant Antibody (HPAB-0173-CN) | ELISA | Humanized IgG1, κ |
| HPAB-0175-CN | Human Anti-FAP Recombinant Antibody (clone ESC14) | ELISA, WB, FC, IHC, IP | Human IgG1 |
| HPAB-0668-YJ | Human Anti-FAP Recombinant Antibody (HPAB-0668-YJ) | ELISA, Inhib, IHC, IF, FuncS | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0170-CN-S(P) | Human Anti-FAP Recombinant Antibody; scFv Fragment (HPAB-0170-CN-S(P)) | ELISA, IF | Human scFv |
| HPAB-0171-CN-S(P) | Mouse Anti-FAP Recombinant Antibody; scFv Fragment (HPAB-0171-CN-S(P)) | ELISA | Mouse scFv |
| HPAB-0173-CN-S(P) | Human Anti-FAP Recombinant Antibody; scFv Fragment (HPAB-0173-CN-S(P)) | ELISA | Humanized scFv |
| HPAB-0175-CN-S(P) | Human Anti-FAP Recombinant Antibody (clone ESC14); scFv Fragment | ELISA, WB, FC, IHC, IP | Human scFv |
| HPAB-0668-YJ-S(P) | Human Anti-FAP Recombinant Antibody; scFv Fragment (HPAB-0668-YJ-S(P)) | ELISA, Inhib, IHC, IF, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0170-CN-F(E) | Human Anti-FAP Recombinant Antibody; Fab Fragment (HPAB-0170-CN-F(E)) | ELISA, IF | Human Fab |
| HPAB-0171-CN-F(E) | Mouse Anti-FAP Recombinant Antibody; Fab Fragment (HPAB-0171-CN-F(E)) | ELISA | Mouse Fab |
| HPAB-0172-CN-F(E) | Human Anti-FAP Recombinant Antibody; Fab Fragment (HPAB-0172-CN-F(E)) | ELISA, IHC | Chimeric (mouse/human) Fab |
| HPAB-0176-CN-F(E) | Human Anti-FAP Recombinant Antibody (clone cF19); Fab Fragment | ELISA, FC, IHC, IP, IF | Chimeric (mouse/human) Fab |
| HPAB-0668-YJ-F(E) | Human Anti-FAP Recombinant Antibody; Fab Fragment (HPAB-0668-YJ-F(E)) | ELISA, Inhib, IHC, IF, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0619-CN | Recombinant Dromedary Anti-FAP Single Domain Antibody (HPAB-0619-CN) | ELISA | VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-211 | Afuco™ Anti-FAP ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-211) | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0661 | PE-H-2Kb/Human FAP (SDYYFSWL) MHC Tetramer | FCM, IHC | |
| MHC-CN0662 | APC-H-2Kb/Human FAP (SDYYFSWL) MHC Tetramer | FCM, IHC | |
| MHC-CN0663 | FITC-H-2Kb/Human FAP (SDYYFSWL) MHC Tetramer | FCM, IHC | |
| MHC-CN0664 | H-2Kb/Human FAP (SDYYFSWL) MHC Tetramer | FCM, IHC | |
| MHC-YF573 | H-2Kb/Human muFAP alpha (SDYYFSWL) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY97 | AbPlus™ Anti-FAP Magnetic Beads (ESC14) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY118 | Human Anti-FAP (clone cF19) scFv-Fc Chimera | FC, IHC, IP | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY813 | Anti-FAP Immunohistochemistry Kit | IHC | |
| VS-0525-XY2444 | Anti-Human FAP Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC379 | CytoStream™ Mouse Anti-FAP Recombinant Antibody (VS13-YC379) | IHC-P, FC, ELISA | Mouse IgG1 |
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