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Rabbit Anti-HSPD1 Polyclonal Antibody (MRO-1944-CN) (CAT#: MRO-1944-CN)

This antibody is a rabbit polyclonal antibody that is capable of binding to HSPD1.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
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  • Fc Engineering:
  • Modalities:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/if_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/50025/if_selected.jpg
Figure 3 IHC staining of human kidney
Figure 3 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/ihc_selected.jpg
Figure 4 IHC staining of human rectum
Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2775/ihc_selected.jpg
Figure 5 IHC staining of human colon
Figure 5 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72816/ihc_selected.jpg
Figure 6 IF staining of human cell line U-2 OS
Figure 6 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/29_C6_1_blue_red_green.jpg
Figure 7 Cerebral cortex
Figure 7 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_B_8_5.jpg
Normal Tissue
Figure 8 Colon
Figure 8 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_7_3.jpg
Figure 9 Liver
Figure 9 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_9_4.jpg
Figure 10 Pancreas
Figure 10 Pancreas
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_1_3.jpg
Figure 11 Kidney
Figure 11 Kidney
(Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_8_5.jpg
Figure 12 Testis
Figure 12 Testis
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_6_6.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Figure 13 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1
Figure 13 RNA cell line category: Low cell line specificity
Figure 13 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1

Specifications

  • Immunogen
  • Peptide
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human HSPD1 (Heat Shock Protein Family D (Hsp60) Member 1)
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunohistochemistry; Flow Cytometry
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Immunogen affinity purified
  • Format
  • Liquid
  • Buffer
  • 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
  • Preservative
  • 0.05% Sodium Azide
  • Storage
  • Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Target

  • Alternative Names
  • Heat Shock Protein Family D (Hsp60) Member 1; Heat Shock 60kDa Protein 1 (Chaperonin); Mitochondrial Matrix Protein P1; P60 Lymphocyte Protein; 0 KDa Chaperonin; Chaperonin 60; HuCHA60; HSP-60; HSP60; CPN60; Spastic Paraplegia 13 (Autosomal Dominant);

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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DrMAB-1035 Mouse Anti-HSPD1 Recombinant Antibody (clone YC181); Fab Fragment ELISA Mouse Fab
FAMAB-0119JF-F(E) Mouse Anti-HSPD1 Recombinant Antibody (clone II-13); Fab Fragment WB, ELISA, FuncS Mouse Fab
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Customer Reviews and Q&As

Customer Review Q&As

Submit a review or a question


Perfect for Mitochondrial Studies
I found the Rabbit Anti-HSPD1 Polyclonal Antibody particularly useful for mitochondrial studies due to its specificity in localizing to mitochondria. Its performance in immunofluorescence was remarkable, providing clear insights into mitochondrial distribution within the cell. The high-quality staining helped elucidate protein interactions within mitochondria, vital for my research involving cellular stress responses.

Reliable Cancer Research Tool
The antibody's specificity for HSPD1 made it an indispensable tool for cancer research in my lab. Its precision helped in identifying mitochondrial stress proteins involved in tumor progression. I especially appreciated its consistency across batch orders, ensuring reliable results in our long-term studies on apoptosis in cancer cells.

Great Versatility in Applications
The versatility of the Rabbit Anti-HSPD1 Polyclonal Antibody makes it a must-have in any research lab. It worked well in diverse applications like Western blot and immunohistochemistry, and yielded consistent, reproducible results, especially when exploring mitochondrial protein interactions.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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