Rabbit Anti-HSPD1 Polyclonal Antibody (MRO-1944-CN)

CAT#: MRO-1944-CN

This antibody is a rabbit polyclonal antibody that is capable of binding to HSPD1.

Gene Expression
Figure 1 IF staining of human cell line U-251 MG Figure 2 IF staining of human cell line A-431 Figure 3 IHC staining of human kidney Figure 4 IHC staining of human rectum Figure 5 IHC staining of human colon Figure 6 IF staining of human cell line U-2 OS Figure 7 Cerebral cortex Figure 8 Colon Figure 9 Liver Figure 10 Pancreas Figure 11 Kidney Figure 12 Testis Figure 13 RNA cell line category: Low cell line specificity

Specifications

  • Immunogen
  • Peptide
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human HSPD1 (Heat Shock Protein Family D (Hsp60) Member 1)
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunohistochemistry; Flow Cytometry
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Immunogen affinity purified
  • Format
  • Liquid
  • Buffer
  • 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
  • Preservative
  • 0.05% Sodium Azide
  • Storage
  • Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Target

  • Alternative Names
  • Heat Shock Protein Family D (Hsp60) Member 1; Heat Shock 60kDa Protein 1 (Chaperonin); Mitochondrial Matrix Protein P1; P60 Lymphocyte Protein; 0 KDa Chaperonin; Chaperonin 60; HuCHA60; HSP-60; HSP60; CPN60; Spastic Paraplegia 13 (Autosomal Dominant);
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Breast cancer biomarkers at key points during disease progression
Perfect for Mitochondrial Studies
I found the Rabbit Anti-HSPD1 Polyclonal Antibody particularly useful for mitochondrial studies due to its specificity in localizing to mitochondria. Its performance in immunofluorescence was remarkable, providing clear insights into mitochondrial distribution within the cell. The high-quality staining helped elucidate protein interactions within mitochondria, vital for my research involving cellular stress responses.
Breast cancer biomarkers at key points during disease progression
Reliable Cancer Research Tool
The antibody's specificity for HSPD1 made it an indispensable tool for cancer research in my lab. Its precision helped in identifying mitochondrial stress proteins involved in tumor progression. I especially appreciated its consistency across batch orders, ensuring reliable results in our long-term studies on apoptosis in cancer cells.
Breast cancer biomarkers at key points during disease progression
Great Versatility in Applications
The versatility of the Rabbit Anti-HSPD1 Polyclonal Antibody makes it a must-have in any research lab. It worked well in diverse applications like Western blot and immunohistochemistry, and yielded consistent, reproducible results, especially when exploring mitochondrial protein interactions.

Q&As

  1. What is the primary application of the Rabbit Anti-HSPD1 Polyclonal Antibody?

    A: The Rabbit Anti-HSPD1 Polyclonal Antibody is primarily used for immunoassay techniques such as Western blotting, immunofluorescence, immunohistochemistry, and flow cytometry. Its specificity for the HSPD1 protein makes it an excellent tool for studying protein expression and localization in various tissues.

  2. In which subcellular location can this antibody detect HSPD1?

    A: The antibody detects HSPD1 in the mitochondria, as seen in immunofluorescent staining of human cell lines, where it shows mitochondrial localization. This helps in studying mitochondrial dynamics and functions in different cellular processes.

  3. Can this antibody be used in conjunction with other mitochondrial markers?

    A: Yes, it can be used alongside other mitochondrial markers to provide a comprehensive analysis of mitochondrial health and function in different cell types, especially when assessing mitochondrial dynamics.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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