Rabbit Anti-RelA scFv-Fc Chimera (VS-0425-FY6)
CAT#: VS-0425-FY6
Anti-RelA scFv-Fc is a novel recombinant antibody designed to specifically target the RelA subunit of NF-κB (Nuclear Factor kappa-light-chain-enhancer of activated B cells). The product utilizes a single-chain variable fragment (scFv) fused to an Fc region, combining the advantages of high specificity and affinity of antibody fragments with the extended half-life and stability conferred by the Fc domain. scFv-Fc formats are being explored for various therapeutic applications, including cancer immunotherapy and targeted drug delivery.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/63461/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_7_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_5_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18809_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4264/18466_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000173039-RELA
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human NF-kB p65.
- Host Species
- Rabbit
- Type
- Rabbit IgG, scFv-Fc
- Specificity
- Human RelA
- Species Reactivity
- Human, Mouse
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- Related Disease
- Autoinflammatory Disease, Familial, Behcet-Like 3 and Rela Fusion-Positive Ependymoma
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- RELA Proto-Oncogene, NF-KB Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; Nuclear Factor NF-Kappa-B P65 Subunit; NFKB3; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A
- Gene ID
- 5970
- UniProt ID
- Q04206
- Long Name
- RELA Proto-Oncogene, NF-KB Subunit
- Cellular Localization
- Nucleus, Cytoplasm
- Post Translation Modifications
- Ubiquitinated by RNF182, leading to its proteasomal degradation.
Monomethylated at Lys-310 by SETD6.
Phosphorylation at Ser-311 disrupts the interaction with EHMT1 and promotes transcription factor activity.
Phosphorylation at Ser-75 by herpes simplex virus 1/HHV-1 inhibits NF-kappa-B activation.
Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3 and SIRT2.
S-nitrosylation of Cys-38 inactivates the enzyme activity.
Sulfhydration at Cys-38 mediates the anti-apoptotic activity by promoting the interaction with RPS3 and activating the transcription factor activity.
Sumoylation by PIAS3 negatively regulates DNA-bound activated NF-kappa-B.
Proteolytically cleaved within a conserved N-terminus region required for base-specific contact with DNA in a CPEN1-mediated manner, and hence inhibits NF-kappa-B transcriptional activity.
Modification sites at PhosphoSitePlus
Modification sites at neXtProt
Glycosylation from GlyGen 10 sites, 2 O-linked glycans (10 sites)
- Protein Refseq
- XP_047283348.1; XP_054225577.1; NP_001138610.1; NP_001230913.1; NP_001230914.1
- Function
- NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.
NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52.
The heterodimeric RELA-NFKB1 complex appears to be most abundant one.
The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0425-FY6. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "RELA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B027(A) | Recombinant Anti-human RELA Intrabody [(D-Arg)9] | WB, CO-IP, FuncS | scFv-(D-Arg)9 |
| IAB-B027(G) | Recombinant Anti-human RELA Intrabody [+36 GFP] | IF, ELISA, FuncS | scFv-(+36GFP) |
| IAB-B027(T) | Recombinant Anti-human RELA Intrabody [Tat] | FC, IF, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0722MZ | Chicken Anti-Nuclear factor NFκB, p65 subunit (RELA) Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3043 | Hi-Affi™ Recombinant Rabbit Anti-RELA Monoclonal Antibody (DS3043AB) | FC, IP, WB | IgG |
| MOR-4654 | Hi-Affi™ Recombinant Rabbit Anti-RELA Monoclonal Antibody (TH167DS) | WB, IHC-P, FC, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1092-CN | Recombinant Rabbit Anti-RELA Monoclonal Antibody (CBACN-401) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-2349-CN | Recombinant Rabbit Anti-RELA (phosphorylated Ser529) Monoclonal Antibody (SP07-00) | WB, IP, FC | Rabbit IgG |
| ZG-0313C | Mouse Anti-RELA Recombinant Antibody (ZG-0313C) | WB, ELISA | Mouse IgG |
| VS3-FY1264 | Recombinant Rabbit Anti-RELA Antibody (clone R07-2B6) | WB, ICC, IF | Rabbit IgG |
| VS7-0425-WR472 | Mouse Anti-RELA Monoclonal Antibody (clone 6H7) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY223 | AbPlus™ Anti-RELA Magnetic Beads (CBACN-401) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC998 | CytoStream™ Rabbit Anti-RELA Recombinant Antibody (VS13-YC998) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6072 | Anti-RELA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6073 | Anti-Mouse RELA Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IHC, FuncS, Inhib, Cyt
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: ELISA, Neut, FuncS
-2.png)
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, Inhib, FuncS
Application: FC, IA, IF, IP, IHC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
