What is the clonality and target of this antibody?

This is a Rabbit Polyclonal Antibody that targets Human SCN9A (Sodium Voltage-Gated Channel Alpha Subunit 9). It was generated using a peptide immunogen.

What immunoassay techniques can this antibody be used for?

This antibody is capable of binding to SCN9A in Immunofluorescence (IF), Immunohistochemistry (IHC), and Flow Cytometry (FC). It is purified by peptide affinity purification.

Rabbit Anti-SCN9A Polyclonal Antibody (MRO-2056-CN)

CAT#: MRO-2056-CN

This antibody is a rabbit polyclonal antibody that is capable of binding to SCN9A.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to plasma membrane, actin filaments & focal adhesion sites.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61843/1332_D6_5_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human colon

Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13679/30465_A_7_3_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13679/30465_B_7_5.jpg

Normal Tissue Figure 4 Colon

Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13679/30465_A_7_3.jpg

Normal Tissue Figure 5 Liver

Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13679/30465_A_9_4.jpg

Normal Tissue Figure 6 Kidney

Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13679/30465_A_8_5.jpg

Normal Tissue Figure 7 Testis

Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13679/30465_A_6_6.jpg

Normal Tissue Figure 8 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13679/30465_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, HHSteC, hTERT-RPE1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169432-SCN9A/subcellular

❮

❯

Specifications

Immunogen
Peptide

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human SCN9A (Sodium Voltage-Gated Channel Alpha Subunit 9)

Species Reactivity
Human

Applications
Used for immunoassay techniques such as: Immunofluorescence; Immunohistochemistry; Flow Cytometry

Conjugate
Unconjugated

Product Property

Purification
Peptide affinity purified

Format
Liquid

Buffer
1*TBS, pH 7.4, 0.5% BSA, 50% Glycerol

Preservative
0.05% Sodium Azide

Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Target

Alternative Names
Sodium Voltage-Gated Channel Alpha Subunit 9; Sodium Channel, Voltage-Gated, Type IX, Alpha Polypeptide; Sodium Channel, Voltage-Gated, Type IX, Alpha Subunit; Voltage-Gated Sodium Channel Subunit Alpha Nav1.7; Sodium Channel Protein Type IX Subunit Alpha; Neuroendocrine Sodium Channel; Peripheral Sodium Channel 1; HNE-Na; NENA; PN1; Sodium Channel, Voltage Gated, Type IX Alpha Subunit;

Gene ID
6335

UniProt ID
Q15858

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Breast cancer biomarkers at key points during disease progression

Effective Immunofluorescence

We used this rabbit polyclonal antibody for immunofluorescence imaging of SCN9A in neuronal cells. It provided a bright signal, helping us visualize the sodium channel distribution. The peptide affinity purification seems to provide good specificity for the target protein in our assays.

Reliable For Flow Cytometry

We validated this antibody in flow cytometry to analyze surface expression of SCN9A. It distinguished our positive population effectively. As a polyclonal antibody, it seems to have robust binding, and we successfully used it to study nociception signaling pathways in human cells.

Q&As

What is the clonality and target of this antibody?

A: This is a Rabbit Polyclonal Antibody that targets Human SCN9A (Sodium Voltage-Gated Channel Alpha Subunit 9). It was generated using a peptide immunogen.
What immunoassay techniques can this antibody be used for?

A: This antibody is capable of binding to SCN9A in Immunofluorescence (IF), Immunohistochemistry (IHC), and Flow Cytometry (FC). It is purified by peptide affinity purification.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MRO-2056-CN.pdf

MSDS

MSDS - MRO-2056-CN.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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