Rabbit Anti-SP1 (phospho T739) Antibody
CAT#: VS-0126-XL1286
Rabbit polyclonal recognizing the transcription factor SP1 phosphorylated at Threonine 739. This marker identifies regulatory modifications influencing gene expression, optimized specifically for WB detection.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12292/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_6_2_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12292/95_H4_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1853/7530_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185591-SP1
❮
❯
Specifications
- Host Species
- Rabbit
- Specificity
- SP1
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Site-selective antibodies were isolated via phosphopeptide matrices, with non-phospho binders eliminated through subtractive elution from unmodified peptide columns.
- Concentration
- 1.0 mg/mL
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- Predicted MW: 90 kDa
WB: 1:500-1:1000
Target
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Related Signaling Pathways
MAPK Signaling Pathway
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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