Recombinant Rabbit Anti-ABCB1 Antibody (clone R02-6B2)
CAT#: VS3-FY5
This product is a recombinant rabbit antibody that recognizes human ABCB1 protein. It can detect a band of approximately 141 kDa (observedd molecular weight: 130-180 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2199/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74614/1893_J14_31_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/203_B4_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_B_9_5.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1716/4431_A_9_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (Hep G2, RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085563-ABCB1
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WB
Figure 1. Recombinant Rabbit Anti-ABCB1 Antibody (clone R02-6B2) in WB.
Western blot analysis of P-glycoprotein from HeLa lysates using P-glycoprotein antibody.
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Specifications
- Immunogen
- Recombinant protein of human P Glycoprotein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ABCB1
- Species Reactivity
- Human
- Clone
- R02-6B2
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 141 kDa; Observed MW: 130-180 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- ATP Binding Cassette Subfamily B Member 1; ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1; Multidrug Resistance Protein 1; Colchicin Sensitivity; P-Glycoprotein 1; EC 3.6.3.44; MDR1; PGY1; ATP-Binding Cassette Sub-Family B Member 1; Doxorubicin Resistance
- Gene ID
- 5243
- UniProt ID
- P08183
- Sequence Similarities
- Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
- Cellular Localization
- Cell membrane, Membrane
- Protein Refseq
- NP_000918.2
- Function
- Translocates drugs and phospholipids across the membrane (PubMed:8898203, PubMed:2897240, PubMed:9038218).
Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203).
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:9038218).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ABCB1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22-F(E) | Recombinant Anti-human ABCB1 Antibody Fab Fragment | FC, WB, FuncS | Fab |
| MHH-22-F(E) | Recombinant Human Anti-human ABCB1 Antibody Fab Fragment | WB, FC, FuncS | Fab |
| PFBC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219); Fab Fragment | WB, Neut, IF, IHC, IHC-P, IP | Mouse IgG |
| PFBC-444 | Human Anti-ABCB1 Recombinant Antibody Fab Fragment (PFBC-444) | FC, Block, IP, IHC | Human Fab |
| HPAB-M0001-YC-F(E) | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6); Fab Fragment | IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-22-S(P) | Recombinant Anti-human ABCB1 Antibody scFv Fragment | Neut, IF, FuncS | scFv |
| MHH-22-S(P) | Recombinant Human Anti-human ABCB1 Antibody scFv Fragment | FC, IP, FuncS | scFv |
| PSBC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219); scFv Fragment | WB, Neut, IF, IHC, IHC-P, IP | Mouse scFv |
| PSBC-444 | Human Anti-ABCB1 Recombinant Antibody scFv Fragment (PSBC-444) | FC, Block, IP, IHC | Mouse scFv |
| HPAB-M0001-YC-S(P) | Mouse Anti-ABCB1 Recombinant Antibody (clone 6C6); scFv Fragment | IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-052 | Mouse Anti-ABCB1 Recombinant Antibody (clone C219) | WB, IF, IHC, FC, FuncS | Mouse IgG |
| PABC-444 | Human Anti-ABCB1 Recombinant Antibody (PABC-444) | FuncS, FC, Block, IP, IHC | Human IgG1, κ |
| VS3-QX2 | Mouse Anti-ABCB1 Recombinant Antibody (clone 1G6) | WB, IP, IF | Mouse IgG1 |
| VS4-WK1 | Rabbit Anti-ABCB1 Recombinant Antibody (clone 11H10) | ELISA, IHC, IF | Rabbit IgG |
| VS4-WK2 | Mouse Anti-ABCB1 Recombinant Antibody (clone 5C12D3) | ELISA, WB, IF, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-001CQ | Mouse Anti-ABCB1 Recombinant Antibody (clone UIC2) | FC, Block, IP, IHC | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0009 | Hi-Affi™ Rabbit Anti-ABCB1 Recombinant Antibody (clone DS9AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY6 | Anti-ABCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY16 | Anti-Mouse ABCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY15 | Anti-Human ABCB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY17 | Anti-Goat ABCB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC423 | Recombinant Anti-ABCB1 Vesicular Antibody, EV Displayed (VS-0425-YC423) | Block, ELISA, FC, Cell-uptake |
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