Recombinant Rabbit Anti-ACTA1 Antibody (clone R03-5D1) (CAT#: VS3-FY22)
This product is a recombinant rabbit antibody that recognizes human, mouse and rat ACTA1 protein. It can detect a band of approximately 42 kDa (observedd molecular weight: 42 kDa).
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WB
Figure 1. Recombinant Rabbit Anti-ACTA1 Antibody (clone R03-5D1) in WB.
Western blot analysis of actin from K562, rat brain, C6, 3T3, Hela lysates using α-Skeletal Muscle Actin Antibody.
Specifications
- Immunogen
- A synthetic peptide of human Actin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ACTA1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-5D1
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 42 kDa; Observed MW: 42 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Actin, Alpha 1, Skeletal Muscle; Nemaline Myopathy Type 3; ACTA; Actin, Alpha Skeletal Muscle; Alpha-Actin-1; CFTD1; CFTDM; ASMA
- Gene ID
- 58
- UniProt ID
- P68133
- Sequence Similarities
- Belongs to the actin family.
- Cellular Localization
- Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
Methylated at His-75 by SETD3.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515).
The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
- Protein Refseq
- NP_001091.1
- Function
- Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1782MZ | Mouse Anti-ACTA1 Recombinant Antibody (clone 6D6.G9.D8) | IHC-P | Mouse IgM |
| ZG-0181C | Mouse Anti-ACTA1 Recombinant Antibody (clone 4B11) | WB, IHC-P, IF, IP | Mouse IgG |
| VS4-WK11 | Mouse Anti-ACTA1 Recombinant Antibody (VS4-WK11) | ELISA, WB, IP | Mouse IgG |
| VS3-FY2619 | Rabbit Anti-ACTA1 Recombinant Antibody (VS3-FY2619) | WB, IHC, ICC, IP | Rabbit IgG |
| VS7-HM20 | Mouse Anti-ACTA1 Recombinant Antibody (clone 2H3) | IHC-p, IF | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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