Recombinant Rabbit Anti-ACTN4 Antibody (clone R06-3D7)
CAT#: VS3-FY35
This product is a recombinant rabbit antibody that recognizes human, mouse and rat ACTN4 protein. It can detect a band of approximately 105 kDa (observedd molecular weight: 105 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1873/63_D5_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human liver
Immunohistochemical staining of human liver shows strong cytoplasmic and membranous positivity in bile duct cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1873/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1873/7921_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1873/7921_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1873/7921_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1873/7921_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1873/7921_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1873/7921_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A549)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130402-ACTN4
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WB
Figure 1. Recombinant Rabbit Anti-ACTN4 Antibody (clone R06-3D7) in WB.
Western blot analysis of alpha Actinin 4 in K562, rat brain, C6, 3T3, Hela lysates using alpha Actinin 4 Antibody.
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Specifications
- Immunogen
- A synthetic peptide of human alpha Actinin 4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ACTN4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-3D7
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 105 kDa; Observed MW: 105 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ACTN4"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0357CL | Anti-ACTN4 Recombinant Antibody | IHC, ELISA | |
| TAB-0357CL-S(P) | Anti-ACTN4 Recombinant Antibody scFv Fragment | IHC, ELISA | |
| TAB-0651CLV | Mouse Anti-ACTN4 Recombinant Antibody (TAB-0651CLV) | WB, IHC | Mouse IgG |
| TAB-0651CLV-F(E) | Mouse Anti-ACTN4 Recombinant Antibody; Fab Fragment (TAB-0651CLV-F(E)) | WB, IHC | Mouse Fab |
| TAB-0651CLV-S(P) | Mouse Anti-ACTN4 Recombinant Antibody; scFv Fragment (TAB-0651CLV-S(P)) | WB, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0057 | Hi-Affi™ Rabbit Anti-ACTN4 Recombinant Antibody (clone DS57AB) | ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0073-CN | Mouse Anti-ACTN4 Recombinant Antibody (clone N01) | ELISA, IHC | Mouse IgG2 |
| HPAB-0187-LSX | Mouse Anti-ACTN4 Recombinant Antibody (HPAB-0187-LSX) | ELISA, WB, IHC | Mouse IgG |
| HPAB-0188-LSX | Mouse Anti-ACTN4 Recombinant Antibody (HPAB-0188-LSX) | ELISA, WB, IHC | Mouse IgG |
| HPAB-0189-LSX | Mouse Anti-ACTN4 Recombinant Antibody (HPAB-0189-LSX) | ELISA, WB, IHC | Mouse IgG |
| HPAB-0190-LSX | Mouse Anti-ACTN4 Recombinant Antibody (HPAB-0190-LSX) | ELISA, WB, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0187-LSX-S(P) | Mouse Anti-ACTN4 Recombinant Antibody scFv Fragment (HPAB-0187-LSX-S(P)) | ELISA, WB, IHC | Mouse scFv |
| HPAB-0188-LSX-S(P) | Mouse Anti-ACTN4 Recombinant Antibody scFv Fragment (HPAB-0188-LSX-S(P)) | ELISA, WB, IHC | Mouse scFv |
| HPAB-0189-LSX-S(P) | Mouse Anti-ACTN4 Recombinant Antibody scFv Fragment (HPAB-0189-LSX-S(P)) | ELISA, WB, IHC | Mouse scFv |
| HPAB-0190-LSX-S(P) | Mouse Anti-ACTN4 Recombinant Antibody scFv Fragment (HPAB-0190-LSX-S(P)) | ELISA, WB, IHC | Mouse scFv |
| HPAB-0191-LSX-S(P) | Mouse Anti-ACTN4 Recombinant Antibody (clone CBLC-001); scFv Fragment | ELISA, WB, FC, RIA | Mouse IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0187-LSX-F(E) | Mouse Anti-ACTN4 Recombinant Antibody Fab Fragment (HPAB-0187-LSX-F(E)) | ELISA, WB, IHC | Mouse Fab |
| HPAB-0188-LSX-F(E) | Mouse Anti-ACTN4 Recombinant Antibody Fab Fragment (HPAB-0188-LSX-F(E)) | ELISA, WB, IHC | Mouse Fab |
| HPAB-0189-LSX-F(E) | Mouse Anti-ACTN4 Recombinant Antibody Fab Fragment (HPAB-0189-LSX-F(E)) | ELISA, WB, IHC | Mouse Fab |
| HPAB-0190-LSX-F(E) | Mouse Anti-ACTN4 Recombinant Antibody Fab Fragment (HPAB-0190-LSX-F(E)) | ELISA, WB, IHC | Mouse Fab |
| HPAB-0191-LSX-F(E) | Mouse Anti-ACTN4 Recombinant Antibody (clone CBLC-001); Fab Fragment | ELISA, WB, FC, RIA | Mouse IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF314 | A*02:01/Human ACTN4 (K5N) (FIASNGVKLV) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0057JF | Human Anti-ACTN4 Recombinant Antibody (clone A2) | WB, ELISA, IHC, IF | Human IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1368 | AbPlus™ Anti-ACTN4 Magnetic Beads (VS-0724-YC1368) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY125 | Anti-ACTN4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY126 | Anti-Mouse ACTN4 Immunohistochemistry Kit | IHC |
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