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    Recombinant Rabbit Anti-ALYREF Antibody (clone R01-2A4) (CAT#: VS3-FY73)

    This product is a recombinant rabbit antibody that recognizes human, mouse and rat ALYREF protein. It can detect a band of approximately 27 kDa (observedd molecular weight: 27 kDa).

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    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
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    Conjugation :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19799/if_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human gallbladder
    Figure 2 IHC staining of human gallbladder
    (Immunohistochemical staining of human gallbladder shows nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_6_4_selected.jpg
    Figure 3 Lymph node
    Figure 3 Lymph node
    (Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_7_8.jpg
    Figure 4 Cerebral cortex
    Figure 4 Cerebral cortex
    (Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_B_7_5.jpg
    Figure 5 Colon
    Figure 5 Colon
    (Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_7_3.jpg
    Figure 6 Liver
    Figure 6 Liver
    (Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_9_4.jpg
    Figure 7 Kidney
    Figure 7 Kidney
    (Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_8_5.jpg
    Figure 8 Testis
    Figure 8 Testis
    (Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16281/38406_A_6_6.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183684-ALYREF

    Specifications

    • Immunogen
    • A synthetic peptide of human Aly
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse and Rat ALYREF
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • R01-2A4
    • Applications
    • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
    • Conjugate
    • Unconjugated
    • MW
    • Calculated MW: 27 kDa; Observed MW: 27 kDa

    Product Property

    • Purification
    • Affinity Purified
    • Purity
    • >95%
    • Format
    • Liquid
    • Buffer
    • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
    • Preservative
    • 0.01% Sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/1000
      Immunohistochemistry: 1/50-1/100
      Immunofluorescence: 1/50-1/200
      Immunoprecipitation: 1/20

    Target

    • Alternative Names
    • Aly/REF Export Factor; Transcriptional Coactivator Aly/REF; BZIP-Enhancing Factor BEF; Ally Of AML-1 And LEF-1; THO Complex 4; THOC4; Tho4

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "ALYREF"

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    IgG Antibodies IHC Kits & Antibodies
    CAT Product Name Application Type
    MOB-1890z Mouse Anti-ALYREF Recombinant Antibody (clone 11H8) WB, ELISA, ICC, IF, IP Mouse IgG1
    VS1-YJ7 Mouse Anti-ALYREF Recombinant Antibody (clone 11G5) FC, IHC-P, ICC, ELISA Mouse IgG1

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