Recombinant Mouse Anti-AMACR Antibody (VS3-FY74)
CAT#: VS3-FY74
This product is a recombinant mouse antibody that recognizes human, mouse and rat AMACR. The antibody detects endogenous AMCAR/P504S proteins protein. The mAb was purified by affinity chromatography purified protein. It can detect a band of approximately 42 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20912/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19527/48457_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19527/48457_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19527/48457_A_8_4.jpg
Normal Tissue
Figure 5 Pancreas
Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19527/48457_A_2_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19527/48457_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19527/48457_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000242110-AMACR
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- The antibody detects endogenous AMCAR/P504S proteins.
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry
- Conjugate
- Unconjugated
- MW
- 42 kDa
Product Property
- Purification
- Affinity Chromatography Purified
- Purity
- >95%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50% Glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western blotting: 1/1000
Immunohistochemistry: 1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "AMACR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1622z | Mouse Anti-AMACR Recombinant Antibody (clone 7B6) | WB, ELISA, IF, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0259MZ | Mouse Anti-AMACR Recombinant Antibody (clone 14I5) | IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0160 | Hi-Affi™ Rabbit Anti-AMACR Recombinant Antibody (clone DS160AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0088-CN | Mouse Anti-AMACR Recombinant Antibody (clone 6F5) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1645-CN | Rabbit Anti-AMACR Polyclonal Antibody (MRO-1645-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0570-YJ | Mouse Anti-AMACR Recombinant Antibody (clone 4A12) | ELISA, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0570-YJ-S(P) | Mouse Anti-AMACR Recombinant Antibody (clone 4A12); scFv Fragment | ELISA, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0571-YJ-S(P) | Mouse Anti-AMACR Recombinant Antibody (clone ch4A12); scFv Fragment | ELISA, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0570-YJ-F(E) | Mouse Anti-AMACR Recombinant Antibody (clone 4A12); Fab Fragment | ELISA, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0571-YJ-F(E) | Human Anti-AMACR Recombinant Antibody (clone ch4A12); Fab Fragment | ELISA, IHC | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY342 | Anti-AMACR Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY343 | Anti-Mouse AMACR Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY344 | Anti-Rat AMACR Immunohistochemistry Kit | IHC |
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