Recombinant Mouse Anti-AQP4 Antibody (VS3-FY92)
CAT#: VS3-FY92
This product is a recombinant mouse antibody that recognizes human, mouse and rat AQP4. The Aquaporin 4 mouse Monoclonal antibody detects endogenous Aquaporin 4 proteins protein. It can detect a band of approximately 26 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14784/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5079/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14784/141530_B_7_5.jpg
Normal Tissue
Figure 4 Lung
Alveolar cells type I
Staining: High
Intensity: Strong
Quantity: 75%-25%
Alveolar cells type II
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14784/141530_A_3_4.jpg
Normal Tissue
Figure 5 Kidney
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14784/141530_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (AF22, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171885-AQP4
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- The Aquaporin 4 Mouse Monoclonal antibody detects endogenous Aquaporin 4 proteins.
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Western Blot, Immunohistochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- 26 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/1000-1/2000
Immunofluorescence: 1/100-200
Immunohistochemistry:1/50-300
Target
- Alternative Names
- Aquaporin 4; Mercurial-Insensitive Water Channel; MIWC; WCH4; Aquaporin Type4; Aquaporin-4; AQP-4
- Gene ID
- 361
- UniProt ID
- P55087
- Sequence Similarities
- Belongs to the MIP/aquaporin (TC 1.A.8) family.
- Cellular Localization
- Cell membrane, Cell projection, Endosome, Membrane
- Post Translation Modifications
- Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40% (By similarity).
Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated.
- Protein Refseq
- NP_001641.1; NP_004019.1
- Function
- Forms a water-specific channel (PubMed:7559426, PubMed:8601457, PubMed:19383790).
Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY92. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "AQP4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1110z | Mouse Anti-AQP4 Recombinant Antibody (clone 28H5) | WB, IP, IF, IHC, FC | Mouse IgG3 |
| MOB-0672MZ | Mouse Anti-AQP4 Recombinant Antibody (clone 4E3) | ELISA, IHC-Fr, IHC-P, IP, WB | Mouse IgG1 |
| VS3-CJ1042 | Mouse Anti-AQP4 Recombinant Antibody (VS3-CJ1042) | WB, ICC, IF, IHC | Mouse IgG |
| VS4-WK34 | Mouse Anti-AQP4 Recombinant Antibody (VS4-WK34) | ELISA, WB, IHC, IF | Mouse IgG |
| VS3-XY3264 | Rabbit Anti-AQP4 Recombinant Antibody (clone R05-8C3) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0977CLV | Anti-Human AQP4 Recombinant Antibody (D15107) | ELISA, FuncS, WB, IF, FC | Chimeric antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0223 | Hi-Affi™ Rabbit Anti-AQP4 Recombinant Antibody (clone DS223AB) | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC282 | Recombinant Anti-AQP4 Vesicular Antibody, EV Displayed (VS-0425-YC282) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY479 | Anti-AQP4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY480 | Anti-Mouse AQP4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY481 | Anti-Rat AQP4 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: Block, Cyt, FuncS, Inhib
Application: ELISA
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
