Recombinant Rabbit Anti-BUB1B Antibody (clone R01-4C1) (CAT#: VS3-FY171)
This product is a recombinant rabbit antibody that recognizes human BUB1B protein. It can detect a band of approximately 120 kDa (observedd molecular weight: 120 kDa).
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8419/68_H6_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8419/68_H6_1_selected.jpg
Figure 2 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156970-BUB1B
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156970-BUB1B
Specifications
- Immunogen
- A synthetic peptide of human BubR1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BUB1B
- Species Reactivity
- Human
- Clone
- R01-4C1
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 120 kDa; Observed MW: 120 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- BUB1 Mitotic Checkpoint Serine/Threonine Kinase B; MAD3/BUB1-Related Protein Kinase; Mitotic Checkpoint Kinase MAD3L; HBUBR1; BUBR1; MAD3L; SSK1; Budding Uninhibited By Benzimidazoles 1 (Yeast Homolog), Beta; Budding Uninhibited By Benzimidazoles 1 Homolog Beta (Yeast)
- Gene ID
- 701
- UniProt ID
- O60566
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BUB1B"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0945MZ | Mouse Anti-BUB1B Recombinant Antibody (clone CBLS-180) | IHC-P | Mouse IgG |
| MRO-1702-CN | Rabbit Anti-BUB1B Polyclonal Antibody (MRO-1702-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0575J | Rabbit Anti-BUB1B Recombinant Antibody (clone 7H4) | ELISA, WB, FC | Rabbit IgG |
| VS-0422-LC134 | Mouse Anti-BUB1B Recombinant Antibody (clone mN129A/6R) | ICC, WB | Mouse IgG2a |
| VS-0422-LC135 | Human Anti-BUB1B Recombinant Antibody (clone hN129A/6R) | ICC, WB | Human IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0396 | Hi-Affi™ Rabbit Anti-BUB1B Recombinant Antibody (clone DS396AB) | WB, IHC-P, FC, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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