Recombinant Rabbit Anti-CASP7 Antibody (clone R03-0F2)
CAT#: VS3-FY196
This product is a recombinant rabbit antibody that recognizes human CASP7 protein. It can detect a band of approximately 34 kDa (observedd molecular weight: 34 kDa).
Gene Expression
Normal Tissue
Figure 1 Small intestine
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25563/59600_A_4_2.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25563/59600_A_7_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25563/59600_A_9_5.jpg
Normal Tissue
Figure 4 Testis
Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25563/59600_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25563/59600_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165806-CASP7
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Specifications
- Immunogen
- A synthetic peptide of human pro Caspase-7
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CASP7
- Species Reactivity
- Human
- Clone
- R03-0F2
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 34 kDa; Observed MW: 34 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Caspase 7; Caspase 7, Apoptosis-Related Cysteine Peptidase; Caspase 7, Apoptosis-Related Cysteine Protease; ICE-Like Apoptotic Protease 3; ICE-LAP3; CASP-7; CMH-1; MCH3
- Gene ID
- 840
- UniProt ID
- P55210
- Sequence Similarities
- Belongs to the peptidase C14A family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Post Translation Modifications
- Cleavage by different proteases, such as granzyme B (GZMB), caspase-1 (CASP1), caspase-8 (CASP8), caspase-9 (CASP9) or caspase-10 (CASP10) generate the two active subunits (PubMed:9852092, PubMed:12824163, PubMed:16352606, PubMed:16916640, PubMed:35338844, PubMed:35446120).
Its involvement in different programmed cell death processes is probably specified by the protease that activates CASP7 (PubMed:9852092, PubMed:16916640).
Cleaved and activated by initiator caspases (CASP8, CASP9 and/or CASP10), leading to execution phase of apoptosis (PubMed:16352606, PubMed:16916640, PubMed:21555521, PubMed:27889207).
Cleavage and maturation by GZMB regulates granzyme-mediated programmed cell death (By similarity).
Cleaved and activated by CASP1 in response to bacterial infection (By similarity).
Propeptide domains can also be cleaved efficiently by CASP3 (PubMed:8755496).
Active heterodimers between the small subunit of caspase-7 and the large subunit of CASP3, and vice versa, also occur (PubMed:8755496).
Also cleaved at the N-terminus at alternative sites by CAPN1, leading to its activation (PubMed:19617626).
Phosphorylation at Ser-30 and Ser-239 by PAK2 inhibits its activity (PubMed:21555521, PubMed:27889207).
Phosphorylation at Ser-30 prevents cleavage and activation by initiator caspase CASP9, while phosphorylation at Ser-239 prevents thiol protease activity by preventing substrate-binding (PubMed:21555521, PubMed:27889207).
(Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP7 processing, preventing CASP7 activation and ability to recognize and cleave substrates.
- Protein Refseq
- NP_001218.1; NP_001253985.1; NP_001253986.1
- Function
- Thiol protease involved in different programmed cell death processes, such as apoptosis, pyroptosis or granzyme-mediated programmed cell death, by proteolytically cleaving target proteins.
Has a marked preference for Asp-Glu-Val-Asp (DEVD) consensus sequences, with some plasticity for alternate non-canonical sequences.
Its involvement in the different programmed cell death processes is probably determined by upstream proteases that activate CASP7 (By similarity).
Acts as an effector caspase involved in the execution phase of apoptosis: following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of proteins, such as CLSPN, PARP1, PTGES3 and YY1.
Compared to CASP3, acts as a minor executioner caspase and cleaves a limited set of target proteins (PubMed:18723680).
Acts as a key regulator of the inflammatory response in response to bacterial infection by catalyzing cleavage and activation of the sphingomyelin phosphodiesterase SMPD1 in the extracellular milieu, thereby promoting membrane repair (PubMed:21157428).
Regulates pyroptosis in intestinal epithelial cells: cleaved and activated by CASP1 in response to S.typhimurium infection, promoting its secretion to the extracellular milieu, where it catalyzes activation of SMPD1, generating ceramides that repair membranes and counteract the action of gasdermin-D (GSDMD) pores (By similarity).
Regulates granzyme-mediated programmed cell death in hepatocytes: cleaved and activated by granzyme B (GZMB) in response to bacterial infection, promoting its secretion to the extracellular milieu, where it catalyzes activation of SMPD1, generating ceramides that repair membranes and counteract the action of perforin (PRF1) pores (By similarity).
Following cleavage by CASP1 in response to inflammasome activation, catalyzes processing and inactivation of PARP1, alleviating the transcription repressor activity of PARP1 (PubMed:22464733).
Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (By similarity).
Cleaves and activates sterol regulatory element binding proteins (SREBPs) (PubMed:8643593).
Cleaves phospholipid scramblase proteins XKR4, XKR8 and XKR9 (By similarity).
In case of infection, catalyzes cleavage of Kaposi sarcoma-associated herpesvirus protein ORF57, thereby preventing expression of viral lytic genes (PubMed:20159985).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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