Recombinant Rabbit Anti-CBL Antibody (clone R01-9G1)
CAT#: VS3-FY206
This product is a recombinant rabbit antibody that recognizes human CBL protein. It can detect a band of approximately 100 kDa (observedd molecular weight: 120 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/257_E11_2_selected.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_A_9_8.jpg
Normal Tissue
Figure 3 Tonsil
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_A_4_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Low Intensity: Weak Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Round or early spermatids Staining: Low Intensity: Weak Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27956/61641_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110395-CBL
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ICC
Figure 1. Recombinant Rabbit Anti-CBL Antibody (clone R01-9G1) in ICC.
Immunocytochemical analysis of CBL (green) in K562 using CBL antibody and DAPI (blue).
WB
Figure 2. Recombinant Rabbit Anti-CBL Antibody (clone R01-9G1) in WB.
Western blot analysis of CBL from HeLa lysates using CBL Antibody.
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Specifications
- Immunogen
- A synthetic peptide of human CBL
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CBL
- Species Reactivity
- Human
- Clone
- R01-9G1
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 100 kDa; Observed MW: 120 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cbl Proto-Oncogene; Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence; Cbl Proto-Oncogene, E3 Ubiquitin Protein Ligase; Casitas B-Lineage Lymphoma Proto-Oncogene; RING-Type E3 Ubiquitin Transferase CBL; Signal Transduction Protein CBL; RING Finger Protein 55; Proto-Oncogene C-Cbl; Oncogene CBL2; RNF55
- Gene ID
- 867
- UniProt ID
- P22681
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Golgi apparatus, Membrane
- Post Translation Modifications
- Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70 (By similarity).
Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not phosphorylated at Tyr-731 by FGFR3. Phosphorylated on tyrosine residues by activated CSF1R, PDGFRA and PDGFRB. Phosphorylated on tyrosine residues by HCK.
Ubiquitinated, leading to its degradation via the proteasome (PubMed:11896602, PubMed:20094046).
Ubiquitination is negatively regulated by IFT20 (PubMed:29237719).
- Protein Refseq
- NP_005179.2
- Function
- Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome (PubMed:17094949).
Ubiquitinates SPRY2 (PubMed:17094949, PubMed:17974561).
Ubiquitinates EGFR (PubMed:17974561).
Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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|---|---|---|---|
| MOR-0489 | Hi-Affi™ Rabbit Anti-CBL Recombinant Antibody (clone DS489AB) | FC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| FAMAB-0273-CN | Mouse Anti-CBL Recombinant Antibody (clone 5D1) | WB, ELISA | Mouse IgG |
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| CAT | Product Name | Application | Type |
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| FAMAB-0161-CN-S(P) | Mouse Anti-CBL Recombinant Antibody (clone CBLNC-104); scFv Fragment | WB, ELISA | Mouse scFv |
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| FAMAB-0287-CN-S(P) | Human Anti-CBL Recombinant Antibody (clone CLB-1); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0161-CN-F(E) | Mouse Anti-CBL Recombinant Antibody (clone CBLNC-104); Fab Fragment | WB, ELISA | Mouse Fab |
| FAMAB-0273-CN-F(E) | Mouse Anti-CBL Recombinant Antibody (clone 5D1); Fab Fragment | WB, ELISA | Mouse Fab |
| FAMAB-0287-CN-F(E) | Human Anti-CBL Recombinant Antibody (clone CLB-1); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY67 | Rabbit Anti-NHP CBL Recombinant Antibody (VS-1024-XY67) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY322 | Anti-CBL Immunohistochemistry Kit | IHC | |
| VS-0525-XY1031 | Anti-Human CBL Immunohistochemistry Kit | IHC |
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