Recombinant Mouse Anti-CCL2 Capture Antibody (clone 4A2)
CAT#: VS3-WK1674
This antibody is a mouse recombinant antibody against human CCL2. The sandwich ELISA was established using matched antibody pair 4A2 and 6E1. The clone 4A2 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human CCL2 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG2b.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/1742_B4_8_cr580492c5c7769_selected.jpg
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Specifications
- Immunogen
- Recombinant human CCL2 protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human CCL2
- Species Reactivity
- Human
- Clone
- 4A2
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
- Gene ID
- 6347
- UniProt ID
- P13500
- Long Name
- C-C motif chemokine ligand 2
- Sequence Similarities
- Belongs to the intercrine beta (chemokine CC) family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Processing at the N-terminus can regulate receptor and target cell selectivity.
Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
- Protein Refseq
- NP_002973.1
- Function
- Acts as a ligand for C-C chemokine receptor CCR2.
Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions.
Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils.
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Related Diseases
Rheumatoid Arthritis
Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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