Recombinant Rabbit Anti-CCL2 Detection Antibody (clone R08-2Y4) (CAT#: VS3-WK1689)
This antibody is a rabbit recombinant antibody against human CCL2. It was developed by immunization of rabbit with recombinant human CCL2 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R08-5X2 and R08-2Y4 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R08-2Y4 can be used in ELISA analysis to identify human CCL2.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/1742_B4_8_cr580492c5c7769_selected.jpg
(Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/1742_B4_8_cr580492c5c7769_selected.jpg
Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in dot like pattern in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in dot like pattern in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/ihc_selected.jpg
Figure 3 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_2_2_selected.jpg
(Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_2_2_selected.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/182_D1_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19163/182_D1_1_blue_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_B_9_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_B_9_5.jpg
Figure 6 Colon
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_8_3.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_8_3.jpg
Figure 7 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_7_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13676/29298_A_7_5.jpg
Figure 8 RNA cell line category: Group enriched (BJ hTERT+, HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
Figure 8 RNA cell line category: Group enriched (BJ hTERT+, HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108691-CCL2
Specifications
- Immunogen
- Recombinant human CCL2 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CCL2
- Species Reactivity
- Human
- Clone
- R08-2Y4
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
- Gene ID
- 6347
- UniProt ID
- P13500
- Long Name
- C-C motif chemokine ligand 2
- Sequence Similarities
- Belongs to the intercrine beta (chemokine CC) family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Processing at the N-terminus can regulate receptor and target cell selectivity.
Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
- Protein Refseq
- NP_002973.1
- Function
- Acts as a ligand for C-C chemokine receptor CCR2.
Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions.
Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils.
Recommended Products
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Isotype Control
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Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-038 | Recombinant Anti-Human CCL2 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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