Recombinant Rabbit Anti-CCNA2 Antibody (clone R02-9B6)
CAT#: VS3-FY211
This product is a recombinant rabbit antibody that recognizes human, mouse and rat CCNA2 protein. It can detect a band of approximately 49 kDa (observedd molecular weight: 49 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/667_H11_1_selected.jpg
Normal Tissue
Figure 2 Tonsil
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_6_8_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes - Goblet cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_7_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/114/162272_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145386-CCNA2
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WB
Figure 1. Recombinant Rabbit Anti-CCNA2 Antibody (clone R02-9B6) in WB.
Western blot analysis of cyclin A2 in K562, C6, 3T3, Hela lysates using cyclin A2 antibody.
ICC
Figure 2. Recombinant Rabbit Anti-CCNA2 Antibody (clone R02-9B6) in ICC.
Immunocytochemical analysis of cyclin A2 (green) in HeLa using cyclin A2 antibody and DAPI (blue).
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Specifications
- Immunogen
- A synthetic peptide of human Cyclin A2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CCNA2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-9B6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 49 kDa; Observed MW: 49 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cyclin A2; Cyclin-A; CCN1; CCNA; Cyclin-A2; Cyclin A
- Gene ID
- 890
- UniProt ID
- P20248
- Sequence Similarities
- Belongs to the cyclin family. Cyclin AB subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.
- Protein Refseq
- NP_001228.1
- Function
- Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CCNA2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3730z | Mouse Anti-CCNA2 Recombinant Antibody (clone 1F1) | WB, IHC, IF, ELISA | Mouse IgG2a |
| MOB-2072MZ | Mouse Anti-CCNA2 Recombinant Antibody (clone 3223D3b) | WB | Mouse IgG1 |
| VS3-XY179 | Mouse Anti-CCNA2 Recombinant Antibody (clone 1A8H2C12) | ELISA, WB, IHC, FC | |
| VS3-FY210 | Recombinant Rabbit Anti-CCNA2 Antibody (clone R02-3H6) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS13-YC146 | Rabbit Anti-CCNA2 Recombinant Antibody (VS13-YC146) | WB, ICC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0513 | Hi-Affi™ Rabbit Anti-CCNA2 Recombinant Antibody (clone DS513AB) | IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4366 | PE-B*40:01/Human CCNA2 (YHEDIHTYL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1313 | AbPlus™ Anti-Ccna2 Magnetic Beads (VS-0724-YC1313) | IP, Protein Purification | |
| VS-0724-YC1370 | AbPlus™ Anti-CCNA2 Magnetic Beads (VS-0724-YC1370) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY145 | Mouse Anti-NHP CCNA2 Recombinant Antibody (clone E23.1) | WB, IHC, IF, IP | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY333 | Anti-CCNA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1079 | Anti-Mouse CCNA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1078 | Anti-Human CCNA2 Immunohistochemistry Kit | IHC |
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