Recombinant Rabbit Anti-CCNA2 Antibody (clone R02-9B6) (CAT#: VS3-FY211)
This product is a recombinant rabbit antibody that recognizes human, mouse and rat CCNA2 protein. It can detect a band of approximately 49 kDa (observedd molecular weight: 49 kDa).
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WB
Figure 1. Recombinant Rabbit Anti-CCNA2 Antibody (clone R02-9B6) in WB.
Western blot analysis of cyclin A2 in K562, C6, 3T3, Hela lysates using cyclin A2 antibody.
ICC
Figure 2. Recombinant Rabbit Anti-CCNA2 Antibody (clone R02-9B6) in ICC.
Immunocytochemical analysis of cyclin A2 (green) in HeLa using cyclin A2 antibody and DAPI (blue).
Specifications
- Immunogen
- A synthetic peptide of human Cyclin A2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CCNA2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-9B6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 49 kDa; Observed MW: 49 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cyclin A2; Cyclin-A; CCN1; CCNA; Cyclin-A2; Cyclin A
- Gene ID
- 890
- UniProt ID
- P20248
- Sequence Similarities
- Belongs to the cyclin family. Cyclin AB subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.
- Protein Refseq
- NP_001228.1
- Function
- Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2072MZ | Mouse Anti-CCNA2 Recombinant Antibody (clone 3223D3b) | WB | Mouse IgG1 |
| MRO-1797-CN | Rabbit Anti-CCNA2 Polyclonal Antibody (MRO-1797-CN) | WB, IHC | Rabbit IgG |
| VS3-CJ764 | Rabbit Anti-CCNA2 Recombinant Antibody (VS3-CJ764) | WB, ICC, IF, IHC | Rabbit IgG |
| VS3-FY210 | Recombinant Rabbit Anti-CCNA2 Antibody (clone R02-3H6) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS7-HM312 | Mouse Anti-CCNA2 Recombinant Antibody (clone 6B4D11) | WB, IHC, FCM | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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