Recombinant Mouse Anti-CD5 Antibody (clone 7H6-10F6-5C10)
CAT#: VS3-FY303
This product is a recombinant mouse antibody that recognizes human CD5 protein. The mAb was purified by affinity chromatography.
Gene Expression
Tested Data
Normal Tissue
Figure 1 Testis
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_5_6.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_7_8.jpg
Normal Tissue
Figure 3 Tonsil
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_5_8.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110448-CD5
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IHC
Figure 1. Recombinant Mouse Anti-CD5 Antibody (clone 7H6-10F6-5C10) in IHC.
Immunohistochemical analysis of paraffin-embedded tonsil using CD5 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthesized peptide derived from human CD5
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CD5
- Species Reactivity
- Human
- Clone
- 7H6-10F6-5C10
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- PBS pH 7.3, 50% Glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/100-1/500
Target
- Alternative Names
- CD5 Molecule; Lymphocyte Antigen T1/Leu-1; CD5 Antigen (P56-62); LEU1; T-Cell Surface Glycoprotein CD5; CD5 Antigen; T1
- Gene ID
- 921
- UniProt ID
- P06127
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-3.
- Protein Refseq
- NP_001333385.1; NP_055022.4N
- Function
- May act as a receptor in regulating T-cell proliferation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
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Isotype Control
- CAT
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