Recombinant Mouse Anti-CD79A Antibody (clone 10A10-10C3-1H9) (CAT#: VS3-FY317)

• Immunogen
• Synthesized peptide derived from human CD79a

• Host Species
• Mouse

• Type
• Mouse IgG2a

• Specificity
• Human CD79A

• Species Reactivity
• Human

• Clone
• 10A10-10C3-1H9

• Applications
• Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• PBS pH 7.3, 50% Glycerol, 0.5% BSA

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry: 1/100-1/500

• Alternative Names
• CD79a Molecule; Membrane-Bound Immunoglobulin-Associated Protein; CD79A Antigen (Immunoglobulin-Associated Alpha); CD79a Molecule, Immunoglobulin-Associated Alpha; Surface IgM-Associated Protein; MB-1 Membrane Glycoprotein; Ig-Alpha

• Gene ID
• 973

• UniProt ID
• P11912

• Cellular Localization
• Cell membrane, Membrane

• Post Translation Modifications
• Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylated by LYN. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
  Arginine methylation in the ITAM domain may interfere with the binding of SYK. It promotes signals leading to B-cell differentiation (By similarity).

• Protein Refseq
• NP_001774.1; NP_067612.1

• Function
• Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.

Secondary Antibody

• 2ND-048CQ
• Goat Anti-Mouse IgG2a Antibody, R-PE

• 2RD-A1464-YJ
• Goat Anti-Mouse IgG2a Secondary antibody

• 2RD-A1564-YJ
• Rabbit Anti-Mouse IgG2a Secondary antibody (Clone RM219)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS