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CEACAM5
Recombinant Mouse Anti-CEACAM5 Antibody (clone 10D8-8G10-2A3)

Recombinant Mouse Anti-CEACAM5 Antibody (clone 10D8-8G10-2A3) (CAT#: VS3-FY368)

This product is a recombinant mouse antibody that recognizes human CEACAM5 protein. The mAb was purified by affinity chromatography.

See more Engineered Antibody products for CEACAM5

Tested Data
Gene Expression
Datasheet
MSDS
COA

IHC

Figure 1. Recombinant Mouse Anti-CEACAM5 Antibody (clone 10D8-8G10-2A3) in IHC.

Figure 1. Recombinant Mouse Anti-CEACAM5 Antibody (clone 10D8-8G10-2A3) in IHC.

Immunohistochemical analysis of paraffin-embedded tonsil using antibody to carcinoembryonic antigen CEA. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

Subcellular Location

Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19758/1660_G5_2_selected.jpg

Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11041/ihc_selected.jpg

Normal Tissue

Figure 3 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Gradient
Gradient: Ascending
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_7_3.jpg

Figure 4 Rectum
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_6_3.jpg

RNA Expression

Figure 5 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthesized peptide derived from human carcinoembryonic antigen(CEA)

Host Species
Mouse

Type
Mouse IgG2b

Specificity
Human CEACAM5

Species Reactivity
Human

Clone
10D8-8G10-2A3

Applications
Immunohistochemistry-Paraffin

Conjugate
Unconjugated

Product Property

Purification
Affinity Chromatography Purified

Purity
>95%

Format
Liquid

Buffer
PBS pH 7.3, 50% Glycerol, 0.5% BSA

Preservative
0.02% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Immunohistochemistry: 1/100-1/500

Target

Alternative Names
Carcinoembryonic Antigen Related Cell Adhesion Molecule 5; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5; Meconium Antigen 100; CEA; Carcinoembryonic Antigen; CD66e Antigen; CD66e

Gene ID
1048

UniProt ID
P06731

Sequence Similarities
Belongs to the immunoglobulin superfamily. CEA family.

Cellular Localization
Cell membrane, Membrane

Post Translation Modifications
Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.

Protein Refseq
NP_001278413.1; NP_001295327.1; NP_004354.3

Function
Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:2803308, PubMed:10910050, PubMed:10864933).
Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308).
Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050).
(Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.

Secondary Antibody

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CEACAM5"

Single-domain Antibody

CAT	Product Name	Application	Type
NABL-080	Recombinant Anti-human CEACAM5 VHH Single Domain Antibody	WB, ELISA, IHC, FC, FuncS	Llama VHH
HPAB-0204-FY	Recombinant llama Anti-CEACAM5 Antibody (11C12)	ADCC, ELISA	llama VHH
HPAB-0205-FY	Recombinant llama Anti-CEACAM5 Antibody (15E7)	ADCC, E	llama VHH
HPAB-0206-FY	Recombinant llama Anti-CEACAM5 Antibody (2C2)	ADCC, E	llama VHH
HPAB-1024WJ	Recombinant Camelid Anti-CEACAM5 Single Domain Antibody	SPR, Inhib	Camelid VHH

Mouse Antibody

CAT	Product Name	Application	Type
TAB-703	Anti-Human CEACAM5 Recombinant Antibody (Altumomab)	IF, IP, Neut, FuncS, ELISA, FC, IHC	IgG1
TAB-418LC-F(E)	Anti-Human CEACAM5 Recombinant Antibody Fab Fragment (COL-Human 9)	FC, WB
TAB-419LC-F(E)	Anti-Human CEACAM5 Recombinant Antibody Fab Fragment (COL-Human 12)	FC, WB
TAB-420LC-F(E)	Anti-Human CEACAM5 Recombinant Antibody Fab Fragment (COL-Human 13)	FC, WB
TAB-421LC-F(E)	Anti-Human CEACAM5 Recombinant Antibody Fab Fragment (COL-Human 15)	FC, WB

Immunotoxin

CAT	Product Name	Application
AGTO-L003E	anti-CEACAM5 immunotoxin BDI-1 (IgG)-PE	Cytotoxicity assay, Functional assay
AGTO-L003R	anti-CEACAM5 immunotoxin BDI-1 (IgG)-RTA	Cytotoxicity assay, Functional assay
AGTO-L004E	anti-CEACAM5 immunotoxin BDI-1 (scFv)-PE	Cytotoxicity assay, Functional assay
AGTO-L004R	anti-CEACAM5 immunotoxin BDI-1 (scFv)-RTA	Cytotoxicity assay, Functional assay

Chimeric Antibody

CAT	Product Name	Application	Type
TAB-409LC	Human Anti-CEACAM5 Recombinant Antibody (TAB-409LC)	SPR, ELISA, FC	Humanized antibody
TAB-412LC	Human Anti-CEACAM5 Recombinant Antibody (TAB-412LC)	ELISA, FC	Chimeric (Mouse/Human) IgG1
TAB-409LC-S(P)	Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-409LC-S(P))	SPR, ELISA, FC	Humanized scFv
TAB-412LC-S(P)	Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-412LC-S(P))	FC, ELISA	Human scFv
TAB-413LC-S(P)	Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-413LC-S(P))	FC, ELISA	Human scFv

Human Antibody

CAT	Product Name	Application	Type
TAB-411LC-S(P)	Human Anti-Human CEACAM5 Recombinant Antibody scFv Fragment	ELISA	Human antibody
TAB-411LC-F(E)	Human Anti-Human CEACAM5 Recombinant Antibody Fab Fragment	ELISA	Human antibody

Humanized Antibody

CAT	Product Name	Application	Type
TAB-414LC-S(P)	Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-414LC-S(P))	FC, ELISA	Human scFv
TAB-414LC-F(E)	Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-414LC-F(E))	FC, ELISA	Humanized Fab
TAB-415LC-F(E)	Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-415LC-F(E))	FC, ELISA	Humanized Fab
TAB-416LC-F(E)	Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-416LC-F(E))	FC, ELISA	Humanized Fab
TAB-417LC-F(E)	Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-417LC-F(E))	IF, ELISA	Humanized Fab

Recombinant Antibody

CAT	Product Name	Application	Type
MOB-0089MZ	Recombinant Mouse Anti-Carcinoembryonic antigen Antibody (clone A9203)	ELISA	Mouse antibody
HPAB-0155-FY	Mouse Anti-CEACAM5 Recombinant Antibody (HPAB-0155-FY)	Activ, FuncS	Humanized IgG1, κ
ZG-1071F	Mouse Anti-CEACAM5 Recombinant Antibody (clone PARLAM 4)	FC, ICC, IHC-Fr, IHC-P, WB	Mouse IgG1
VS3-WK1280	Mouse Anti-CEACAM5 Recombinant Antibody (VS3-WK1280)	ELISA, WB	Mouse IgG1
VS4-WK104	Mouse Anti-CEACAM5 Recombinant Antibody (VS4-WK104)	ELISA, IHC	Mouse IgG

Chicken IgY Antibody

CAT	Product Name	Application	Type
BRD-0118MZ	Chicken Anti-CEACAM5 Polyclonal IgY	WB	Chicken antibody

Rabbit Monoclonal Antibody

CAT	Product Name	Application	Type
MOR-0649	Hi-Affi™ Rabbit Anti-CEACAM5 Recombinant Antibody (clone DS649AB)	IHC	Rabbit IgG

scFv Fragment Antibody

CAT	Product Name	Application	Type
HPAB-0155-FY-S(P)	Mouse Anti-CEACAM5 Recombinant Antibody; scFv Fragment (HPAB-0155-FY-S(P))	Activ	Humanized scFv
HPAB-0243-FY-S(P)	Mouse Anti-CEACAM5 Recombinant Antibody (clone M5A); scFv Fragment	ELISA	Mouse scFv
HPAB-0348-YJ-S(P)	Mouse Anti-CEACAM5 Recombinant Antibody (clone 10E1); scFv Fragment	ELISA, IHC	Mouse scFv
HPAB-1094WJ-S(P)	Mouse Anti-CEACAM5 Recombinant Antibody; scFv Fragment (HPAB-1094WJ-S(P))	ELISA, FC	Mouse scFv
HPAB-2106-FY-S(P)	Human Anti-CEACAM5 Recombinant Antibody (clone CEA6); scFv Fragment	ELISA, FC, Inhib	Human scFv

Fab Fragment Antibody

CAT	Product Name	Application	Type
HPAB-0155-FY-F(E)	Mouse Anti-CEACAM5 Recombinant Antibody; Fab Fragment (HPAB-0155-FY-F(E))	Activ	Humanized Fab
HPAB-0243-FY-F(E)	Human Anti-CEACAM5 Recombinant Antibody (clone M5A); Fab Fragment	ELISA	Chimeric (mouse/human) Fab
HPAB-0348-YJ-F(E)	Mouse Anti-CEACAM5 Recombinant Antibody (clone 10E1); Fab Fragment	ELISA, IHC	Mouse Fab
HPAB-1094WJ-F(E)	Mouse Anti-CEACAM5 Recombinant Antibody; Fab Fragment (HPAB-1094WJ-F(E))	ELISA, FC	Mouse Fab
HPAB-2106-FY-F(E)	Human Anti-CEACAM5 Recombinant Antibody (clone CEA6); Fab Fragment	ELISA, FC, Inhib	Human Fab, κ

ADCC Enhanced Antibody

CAT	Product Name	Application	Type
AFC-TAB-703	Afuco™ Anti-CEACAM5 ADCC Recombinant Antibody (Altumomab), ADCC Enhanced	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody

MHC Tetramer for Cancer

CAT	Product Name	Application
MHC-YF353	A*02:01/Human Carcinoembryonic antigen (CEA)-derived peptide CAP1-6D (YLSGADLNL) MHC Pentamer	FCM
MHC-YF354	A*02:01/Human Carcinogenic Embryonic Antigen (CEA) (GVLVGVALI) MHC Pentamer	FCM
MHC-YF355	A*24:02/Human Carcinogenic Embryonic Antigen (CEA) (TYACFVSNL) MHC Pentamer	FCM
MHC-YF360	A*02:01/Human CEA (YLWWVNNQSL) MHC Pentamer	FCM
MHC-YF361	B*07:02/Human CEACAM (LPVSPRLQL) MHC Pentamer	FCM

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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