Recombinant Mouse Anti-CFB Detection Antibody (clone 5E1)
CAT#: VS3-WK1631
This antibody is a mouse recombinant antibody against human CFB. The sandwich ELISA was established using matched antibody pair 1E4 and 5E1. The clone 5E1 can be used in ELISA analysis as a detection antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human CFB protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG1.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/951/170_F11_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1817/1749_A9_28_cr5804bcaa17056_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1817/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_7_3.jpg
Normal Tissue
Figure 7 Rectum
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_4_3.jpg
Normal Tissue
Figure 8 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_7_5.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1832/6333_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, EFO-21, fHDF/TERT166, HSkMC, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000243649-CFB
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Specifications
- Immunogen
- Recombinant human CFB protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CFB
- Species Reactivity
- Human
- Clone
- 5E1
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.01% thimerosal
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Complement Factor B; B-Factor, Properdin; Properdin Factor B; C3/C5 Convertase; EC 3.4.21.47; PBF2; BFD; GBG; BF; Glycine-Rich Beta-Glycoprotein; Glycine-Rich Beta Glycoprotein
- Gene ID
- 629
- UniProt ID
- P00751
- Long Name
- Complement Factor B
- Sequence Similarities
- Belongs to the peptidase S1 family.
- Cellular Localization
- Secreted
- Protein Refseq
- NP_001701.2
- Function
- Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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