Recombinant Rabbit Anti-CLIP1 Antibody (clone R07-1F3)
CAT#: VS3-FY394
This product is a recombinant rabbit antibody that recognizes human CLIP1 protein. It can detect a band of approximately 162 kDa (observedd molecular weight: 170 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to microtubule ends.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26678/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26678/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4399/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26678/56427_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26678/56427_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26678/56427_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26678/56427_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26678/56427_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130779-CLIP1
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IHC
Figure 1. Recombinant Rabbit Anti-CLIP1 Antibody (clone R07-1F3) in IHC.
Immunohistochemical analysis of paraffin-embedded human tonsil using CLIP170 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
ICC
Figure 2. Recombinant Rabbit Anti-CLIP1 Antibody (clone R07-1F3) in ICC.
Immunocytochemical analysis of CLIP17 (green) in HeLa using a CLIP170 antibody and DAPI (blue).
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Specifications
- Immunogen
- A synthetic peptide of human CLIP170
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CLIP1
- Species Reactivity
- Human
- Clone
- R07-1F3
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 162 kDa; Observed MW: 170 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CLIP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2359CT | Recombinant Mouse anti-Human CLIP1 Monoclonal antibody (BG2I2) | ICC, IF, WB, IHC-P |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0729 | Hi-Affi™ Rabbit Anti-CLIP1 Recombinant Antibody (clone DS729AB) | IHC-P, ICC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3427 | PE-B8/Human CLIP1 (ELKVKNLEL) MHC Tetramer | FCM | |
| MHC-LC3428 | APC-B8/Human CLIP1 (ELKVKNLEL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY470 | Anti-CLIP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1516 | Anti-Mouse CLIP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1515 | Anti-Human CLIP1 Immunohistochemistry Kit | IHC |
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