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    Recombinant Mouse Anti-COL18A1 Capture Antibody (clone 12G3) (CAT#: VS3-WK1628)

    This antibody is a mouse recombinant antibody against human COL18A1. The sandwich ELISA was established using matched antibody pair 12G3 and 5E3. The clone 12G3 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human COL18A1 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG1.

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line Hep G2
    Figure 1 IF staining of human cell line Hep G2
    (Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/36104/if_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human liver
    Figure 2 IHC staining of human liver
    (Immunohistochemical staining of human liver shows strong cytoplasmic and extracellular positivity.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_8_4_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Neuropil Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endocrine cells Staining: Medium Intensity: Moderate Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes - Microvilli Staining: Medium Intensity: Moderate Fibroblasts Staining: Medium Intensity: Moderate Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Mucosal lymphoid cells Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Hepatocytes Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_7_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_8_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/30933/168685_A_6_6.jpg
    RNA Expression
    Figure 8 RNA cell line category: Cell line enhanced (BJ hTERT+, EFO-21, NTERA-2, RPTEC TERT1, TIME)
    Figure 8 RNA cell line category: Cell line enhanced (BJ hTERT+, EFO-21, NTERA-2, RPTEC TERT1, TIME)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182871-COL18A1

    Specifications

    • Immunogen
    • Recombinant human COL18A1 protein
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human COL18A1
    • Species Reactivity
    • Human
    • Clone
    • 12G3
    • Applications
    • ELISA
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein G affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.09% sodium azide
    • Storage
    • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • ELISA: 1/1000

    Target

    • Alternative Names
    • Collagen Type XVIII Alpha 1 Chain; Collagen, Type XVIII, Alpha 1; Endostatin; Collagen Alpha-1(XVIII) Chain Isoform 1 Preproprotein; Collagen Alpha-1(XVIII) Chain; Multi-Functional Protein MFP
    • Long Name
    • Collagen Type XVIII Alpha 1 Chain
    • Sequence Similarities
    • Belongs to the multiplexin collagen family.
    • Cellular Localization
    • Basement membrane, Extracellular matrix, Secreted
    • Post Translation Modifications
    • Prolines at the third position of the tripeptide repeating unit (G-X-Y) of the triple-helical regions are hydroxylated.
      Circulating endostatins are found as sialoglycoprotein and asialoglycoprotein structures.
    • Function
    • Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.

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    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "COL18A1"

    Select a product category from the dropdown menu below to view related products.
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    IgG Antibody Products Antibody Magnetic Beads
    CAT Product Name Application Type
    MOB-3275z Mouse Anti-COL18A1 Recombinant Antibody (clone 38B11) WB, IP, IF, ELISA Mouse IgG1, κ
    VS3-WK1629 Recombinant Mouse Anti-COL18A1 Detection Antibody (clone 5E3) ELISA Mouse IgG2a

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